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MASP1

ANTIBODY INFORMATION
 
 

Antibody HPA001617

  

Antibody HPA009641

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA001617   HPA009641  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 2   3  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA001617

  

Antibody HPA009641

  
Immunocytochemistry


Validated
  

Validated
  
Immunohistochemistry

Supported    
Western blot

Uncertain   Uncertain  
Protein array

Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA001617

  

Antibody HPA009641

  

Formal validation: Independent
Validated

Antibody staining overlaps with antibody HPA009641.		  
Validated

Antibody staining overlaps with antibody HPA001617.		  
 

Standard validation

 Supported   Supported  

   

Figure description

 Immunofluorescent staining of human cell line BJ shows localization to nucleoplasm & cytosol.   Immunofluorescent staining of human cell line BJ shows localization to nucleoplasm & cytosol.  

Antibody dilution

 1:45   1:59  

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA001617

  

Antibody HPA009641

  

Standard validation

 Supported    

   

Figure description

 Immunohistochemical staining of human stomach shows distinct positivity in plasma.    

Expression

 RNA: detected in 33 tissues
Protein: detected in 13 cell types		    

Retrieval

 HIER pH6    

Antibody dilution

 1:40    

Literature conformity

 Consistent with extensive gene/protein characterization data.    

RNA consistency

 No internal RNA expression data available for correlation.    

WESTERN BLOT
 
 

Antibody HPA001617

  

Antibody HPA009641

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		  

   

Figure description

 Lane 1: Marker [kDa] 206, 113, 82, 49, 32, 26, 17.8
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: A-431
Lane 5: Liver
Lane 6: Tonsil		    

Target mass (kDa)

 81.9, 79.2, 68.9, 43.6, 40.6   81.9, 68.9  

Antibody dilution

 1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA001617

  

Antibody HPA009641

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA001617

  

Antibody HPA009641

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 121   129  

Antigen sequence

 
PCPYDYIKIKVGPKVLGPFCGEKAPEPISTQSHSVLILFHSDNSGENRGW
RLSYRAAGNECPELQPPVHGKIEPSQAKYFFKDQVLVSCDTGYKVLKDNV
EMDTFQIECLKDGTWSNKIPT
  
TSRVPNDKWFGSGALLSASWILTAAHVLRSQRRDTTVIPVSKEHVTVYLG
LHDVRDKSGAVNSSAARVVLHPDFNIQNYNHDIALVQLQEPVPLGPHVMP
VCLPRLEPEGPAPHMLGLVAGWGISNPNV
 

Matching transcripts

 MASP1-001 - ENSP00000336792 [100%]
MASP1-002 - ENSP00000169293 [100%]
MASP1-003 - ENSP00000296280 [100%]
MASP1-004 - ENSP00000376262 [100%]
MASP1-005 - ENSP00000376264 [100%]
  MASP1-003 - ENSP00000296280 [100%]
MASP1-005 - ENSP00000376264 [100%]
 

Other gene match

    
ANTIGEN VIEW
MASP1-001
MASP1-002
MASP1-003
MASP1-004
MASP1-005
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.