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Immunohistochemical staining of human lateral ventricle shows strong cytoplasmic and nuclear positivity in neuronal cells, neuropil is distinctly stained.
Immunohistochemical staining of human cerebral cortex shows cytoplasmic positivity in neuronal cells.
Expression
RNA: detected in 8 tissues Protein: detected in 9 cell types
RNA: detected in 8 tissues Protein: detected in 40 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:20
1:18
Literature conformity
No avaliable gene/protein characterization data.
No avaliable gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA039504
Antibody HPA056354
Standard validation
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY407066)
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY407066)
Target mass (kDa)
51.7
51.7, 29.4
Antibody dilution
1:125
1:125
PROTEIN ARRAY
Antibody HPA039504
Antibody HPA056354
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
