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Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear membrane.
Immunofluorescent staining of human cell line A-431 shows localization to nuclear membrane & vesicles.
Antibody dilution
1:43
1:111
Literature conformity
The subcellular location is supported by literature.
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA039343
Antibody HPA040048
Standard validation
Approved
Approved
Figure description
Immunohistochemical staining of human testis shows moderate nuclear positivity in seminiferus ducts and leydig cells.
Immunohistochemical staining of human bone marrow shows strong nuclear positivity in bone marrow poietic cells.
Expression
RNA: detected in 37 tissues Protein: detected in 74 cell types
RNA: detected in 37 tissues Protein: detected in 26 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:45
1:75
Literature conformity
Partly consistent with gene/protein characterization data.
Partly consistent with gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA039343
Antibody HPA040048
Standard validation
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Uncertain
No bands detected.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY416958)
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY416958)
Target mass (kDa)
78.1
78.1
Antibody dilution
1:125
1:250
PROTEIN ARRAY
Antibody HPA039343
Antibody HPA040048
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
