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Immunofluorescent staining of human cell line A-431 shows localization to vesicles.
Antibody dilution
1:10
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA031949
Antibody HPA058412
Antibody CAB004415
Antibody CAB078176
Standard validation
Supported
Supported
Figure description
Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic and nuclear positivity in neuronal cells. Neuropil was moderately stained.
Immunohistochemical staining of human cerebral cortex shows distinct positivity in a subset of neuronal cells.
Expression
RNA: detected in 10 tissues Protein: detected in 12 cell types
RNA: detected in 10 tissues Protein: detected in 9 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:150
1:300
Literature conformity
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
RNA consistency
Consistent with RNA expression data.
Mainly consistent with RNA expression data.
Standard validation
Supported
Figure description
Subsets of neuronal cell bodies as well as dendritic processes and synapses were stained in several regions of the brain, most abundant in basal forebrain, hypothalamus and cerebellum.
WESTERN BLOT
Antibody HPA031949
Antibody HPA058412
Antibody CAB004415
Antibody CAB078176
Standard validation
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Supported
Analysis performed using a standard panel of samples. Single band corresponding to the predicted size in kDa (+/-20%).
Uncertain
Analysis performed using a standard panel of samples. No bands detected.
Figure description
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 229, 112, 83.5, 47.9, 32.3, 26.5, 17.2 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
66.9, 47.4, 25.3
66.9, 47.4, 25.3
66.9, 47.4, 25.3, 13.4, 12.7, 9.4, 9.2, 3.8
Antibody dilution
1:250
1:190
1:500
PROTEIN ARRAY
Antibody HPA031949
Antibody HPA058412
Antibody CAB004415
Antibody CAB078176
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
