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GALNT8

ANTIBODY INFORMATION
 
 

Antibody HPA012638

  

Antibody HPA014418

  

Antibody HPA073461

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA012638   HPA014418   HPA073461  

Host species

 Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

   KCNA6 - ENSG00000151079 [100%]
    

Released in version

 4   4   14  

References

      

VALIDATION SUMMARY
 
 

Antibody HPA012638

  

Antibody HPA014418

  

Antibody HPA073461

  
Immunocytochemistry


Validated
    

Validated
  
Immunohistochemistry

Approved   Approved    
Western blot

Supported   Uncertain   Uncertain  
Protein array

Approved   Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA012638

  

Antibody HPA014418

  

Antibody HPA073461

  

Formal validation: Independent
Validated

Antibody staining overlaps with antibody HPA073461.		    
Validated

Antibody staining overlaps with antibody HPA012638.		  
 

Standard validation

 Supported     Supported  

     

Figure description

 Immunofluorescent staining of human cell line U-2 OS shows localization to vesicles.     Immunofluorescent staining of human cell line HEK 293 shows localization to vesicles.  

Antibody dilution

 1:21     1:105  

Literature conformity

 The subcellular location is supported by literature.     The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA012638

  

Antibody HPA014418

  

Antibody HPA073461

  

Standard validation

 Approved   Approved    

     

Figure description

 Immunohistochemical staining of human rectum shows strong cytoplasmic positivity in glandular cells.   Immunohistochemical staining of human pancreas shows moderate cytoplasmic positivity.    

Expression

 RNA: detected in 18 tissues
Protein: detected in 65 cell types		   RNA: detected in 18 tissues
Protein: detected in 72 cell types		    

Retrieval

 HIER pH6   HIER pH6    

Antibody dilution

 1:75   1:250    

Literature conformity

 Consistent with gene/protein characterization data.   Not done.    

RNA consistency

 Mainly not consistent with RNA expression data.   Mainly not consistent with RNA expression data.    

WESTERN BLOT
 
 

Antibody HPA012638

  

Antibody HPA014418

  

Antibody HPA073461

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		  

     

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		     Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 72.9   58.7   72.9  

Antibody dilution

 1:250   1:250   1:350  

PROTEIN ARRAY
 
 

Antibody HPA012638

  

Antibody HPA014418

  

Antibody HPA073461

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:3000   1:4200  

ANTIGEN INFORMATION
 
 

Antibody HPA012638

  

Antibody HPA014418

  

Antibody HPA073461

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 128   44   72  

Antigen sequence

 
NLLDENVCLDQGPFPGNTPIMYYCHEFSSQNVYYHLTGELYVGQLIAEAS
ASDRCLTDPGKAEKPTLEPCSKAAKNRLHIYWDFKPGGAVINRDTKRCLE
MKKDLLGSHVLVLQTCSTQVWEIQHTVR
  
GRGGNNGGVSRVSPVSRGSQEEEEDEDDSYTFHHGITPGEMGTG
  
QMKLFPHSQLFRQWGEDLSEAQQKAAQDLFRKFGYNAYLSNQLPLNRTIP
DTRDYRCLRKTYPSQLPSLSVI
 

Matching transcripts

 GALNT8-001 - ENSP00000252318 [99%]
  GALNT8-201 - ENSP00000408321 [100%]
  GALNT8-001 - ENSP00000252318 [100%]
 

Other gene match

   KCNA6 - ENSG00000151079 [100%]
    
ANTIGEN VIEW
GALNT8-001
GALNT8-201
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.