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PAK4

ANTIBODY INFORMATION
 
 

Antibody HPA070175

  

Antibody HPA072220

  

Antibody CAB025747

  

Antibody CAB044670

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   R&D Systems
   SIGMA
  

Product name

 HPA070175   HPA072220   AF4178   PRS3077  

Host species

 Rabbit   Rabbit   Goat   Rabbit  

Clonality

 pAb   pAb   msAb   msAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity   Affinity  

Other gene match

        

Released in version

 16   16   5   13  

References

        

VALIDATION SUMMARY
 
 

Antibody HPA070175

  

Antibody HPA072220

  

Antibody CAB025747

  

Antibody CAB044670

  
Immunocytochemistry

Approved   Approved      
Immunohistochemistry

    Uncertain   Uncertain  
Western blot

Supported   Supported   Supported   Uncertain  
Protein array

Supported   Supported      

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA070175

  

Antibody HPA072220

  

Antibody CAB025747

  

Antibody CAB044670

  

Standard validation

 Approved   Approved      

       

Figure description

 Immunofluorescent staining of human cell line MCF7 shows localization to plasma membrane & cell junctions.   Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm, plasma membrane & cell junctions.      

Antibody dilution

 1:78   1:45      

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.   The subcellular location is partly supported by literature or no literature is available.      

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA070175

  

Antibody HPA072220

  

Antibody CAB025747

  

Antibody CAB044670

  

Standard validation

     Uncertain   Uncertain  

       

Figure description

     Immunohistochemical staining of human urinary bladder shows strong cytoplasmic positivity in urothelial cells.   Immunohistochemical staining of human colon shows strong cytoplasmic and membranous positivity in glandular cells.  

Expression

     RNA: detected in 37 tissues
Protein: detected in 73 cell types		   RNA: detected in 37 tissues
Protein: detected in 69 cell types		  

Retrieval

     HIER pH6   HIER pH6  

Antibody dilution

     1:25   1:4000  

Literature conformity

     Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.  

RNA consistency

     Mainly not consistent with RNA expression data.   Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA070175

  

Antibody HPA072220

  

Antibody CAB025747

  

Antibody CAB044670

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		  

       

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		    

Target mass (kDa)

 64.1, 54.9, 14.5   64.1   64.1, 54.9, 48.3, 19.6, 16.9, 14.5, 10.1   64.1, 54.9, 48.3, 19.6, 16.9, 14.5, 10.1  

Antibody dilution

 1:260   1:150   1:500   1:500  

PROTEIN ARRAY
 
 

Antibody HPA070175

  

Antibody HPA072220

  

Antibody CAB025747

  

Antibody CAB044670

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		      

       

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.      

Antibody dilution

 1:3100   1:1800      

ANTIGEN INFORMATION
 
 

Antibody HPA070175

  

Antibody HPA072220

  

Antibody CAB025747

  

Antibody CAB044670

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein   Synthetic peptide  

Length (aa)

 56   58      

Antigen sequence

 
RQWQSLIEESARRPKPLVDPACITSIQPGAPKTIVRGSKGAKDGALTLLL
DEFENM
  
TRSNSLRRDSPPPPARARQENGMPEEPATTARGGPGKAGSRGRFAGHSEA
GGGSGDRR
  

											 
																						

											 
																				

																		

										
											
Matching transcripts

												
																					PAK4-003 - ENSP00000351049 [100%] 
PAK4-004 - ENSP00000469413 [100%] 
PAK4-010 - ENSP00000472862 [100%] 
PAK4-011 - ENSP00000326864 [100%] 
PAK4-201 - ENSP00000353625 [100%] 

											 
																						PAK4-003 - ENSP00000351049 [100%] 
PAK4-004 - ENSP00000469413 [100%] 
PAK4-201 - ENSP00000353625 [100%] 

											 
																						
											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
PAK4-003
PAK4-004
PAK4-010
PAK4-011
PAK4-201
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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