Fields »
ABOUT
HELP
BLOG
ZNF337

ANTIBODY INFORMATION
 
 

Antibody HPA044303

  

Antibody HPA064219

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA044303   HPA064219  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 14   14  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA044303

  

Antibody HPA064219

  
Immunocytochemistry

Approved    
Immunohistochemistry

  Uncertain  
Western blot

Supported   Uncertain  
Protein array

Approved   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA044303

  

Antibody HPA064219

  

Standard validation

 Approved    

   

Figure description

 Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear speckles & plasma membrane.    

Antibody dilution

 1:185    

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA044303

  

Antibody HPA064219

  

Standard validation

   Uncertain  

   

Figure description

   Immunohistochemical staining of human cerebellum shows cytoplasmic positivity in Purkinje cells.  

Expression

   RNA: detected in 37 tissues
Protein: detected in 17 cell types		  

Retrieval

   HIER pH6  

Antibody dilution

   1:600  

Literature conformity

   No avaliable gene/protein characterization data.  

RNA consistency

   Not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA044303

  

Antibody HPA064219

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		  

   

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		    

Target mass (kDa)

 86.9   86.9  

Antibody dilution

 1:250   1:380  

PROTEIN ARRAY
 
 

Antibody HPA044303

  

Antibody HPA064219

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:6000   1:4500  

ANTIGEN INFORMATION
 
 

Antibody HPA044303

  

Antibody HPA064219

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 103   76  

Antigen sequence

 
YSHLVSLGIYAEHVLRPKNLGLAHQRQQQLQFSDQSFQSDTAEGQEKEKS
TKPMAFSSPPLRHAVSSRRRNSVVEIESSQGQRENPTEIDKVLKGIENSR
WGA
  
ESSQGQRENPTEIDKVLKGIENSRWGAFKCAERGQDFSRKMMVIIHKKAH
SRQKLFTCRECHQGFRDESALLLHQN
 

Matching transcripts

 ZNF337-001 - ENSP00000365619 [93%]
ZNF337-002 - ENSP00000252979 [93%]
  ZNF337-001 - ENSP00000365619 [100%]
ZNF337-002 - ENSP00000252979 [100%]
 

Other gene match

    
ANTIGEN VIEW
ZNF337-001
ZNF337-002
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.