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Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane & cytosol.
Antibody dilution
1:6
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA007458
Standard validation
Approved
Figure description
Immunohistochemical staining of human hippocampus shows moderate cytopalsmic positivity in neuronal cells, glial cells showed moderate cytoplasmic and nuclear staining.
Expression
RNA: detected in 22 tissues Protein: detected in 79 cell types
Retrieval
HIER pH6
Antibody dilution
1:35
Literature conformity
Consistent with gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA007458
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Current setup is not applicable due to low RNA count.
Figure description
Target mass (kDa)
47, 40.8, 40.4, 38.5, 25, 24.7, 10.4, 9.5
Antibody dilution
1:250
PROTEIN ARRAY
Antibody HPA007458
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
