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Immunofluorescent staining of human cell line A549 shows localization to cytosol & cell junctions.
Antibody dilution
1:5
Literature conformity
The subcellular location is not consistent with literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA015028
Antibody HPA016488
Formal validation: Independent
Validated
Spearman correlation >0.6 for protein expression in tissues using independent antibodies.
Validated
Spearman correlation >0.6 for protein expression in tissues using independent antibodies.
Figure description
Distribution of protein expression (antibody staining). Spearman correlation with HPA016488 across 72 cell types.
Distribution of protein expression (antibody staining). Spearman correlation with HPA015028 across 72 cell types.
Standard validation
Supported
Supported
Figure description
Immunohistochemical staining of human stomach shows strong membranous and cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human stomach shows membranous and cytoplasmic positivity.
Expression
RNA: detected in 35 tissues Protein: detected in 41 cell types
RNA: detected in 35 tissues Protein: detected in 31 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:15
1:25
Literature conformity
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA015028
Antibody HPA016488
Standard validation
Supported
Analysis performed using a standard panel of samples. Single band corresponding to the predicted size in kDa (+/-20%).
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
81.3, 69.8, 22.7
81.3, 69.8
Antibody dilution
1:250
1:250
PROTEIN ARRAY
Antibody HPA015028
Antibody HPA016488
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
