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Immunofluorescent staining of human cell line RH-30 shows localization to nucleoli.
Antibody dilution
1:80
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA003223
Standard validation
Uncertain
Figure description
Immunohistochemical staining of human tonsil shows moderate cytoplasmic positivity in non-germinal center cells and subset of germinal center cells.
Expression
RNA: detected in 3 tissues Protein: detected in 13 cell types
Retrieval
HIER pH6
Antibody dilution
1:50
Literature conformity
No avaliable gene/protein characterization data.
RNA consistency
Not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA003223
Standard validation
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY402993)
Target mass (kDa)
48.8, 45.1, 44.9, 44.4, 42, 40.4
Antibody dilution
1:250
PROTEIN ARRAY
Antibody HPA003223
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
