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Immunofluorescent staining of human cell line U-2 OS shows localization to nucleus & vesicles.
Antibody dilution
1:42
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA055194
Antibody HPA072208
Standard validation
Supported
Supported
Figure description
Immunohistochemical staining of human hair shows strong cytoplasmic positivity in cells in hair cortex.
Immunohistochemical staining of human hair shows strong cytoplasmic positivity in cells in cortex/medulla.
Expression
RNA: detected in 1 tissues Protein: detected in 0 cell types
RNA: detected in 1 tissues Protein: detected in 0 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:2000
1:1000
Literature conformity
Consistent with gene/protein characterization data.
Consistent with gene/protein characterization data.
RNA consistency
No internal RNA expression data available for correlation.
No internal RNA expression data available for correlation.
WESTERN BLOT
Antibody HPA055194
Antibody HPA072208
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Uncertain
Analysis performed using a standard panel of samples. Current setup is not applicable due to low RNA count.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
49.4, 47.2, 46.2, 45.9
49.4
Antibody dilution
1:270
1:140
PROTEIN ARRAY
Antibody HPA055194
Antibody HPA072208
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
