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Immunohistochemical staining of human cerebral cortex shows strong nuclear positivity in neuronal cells.
Immunohistochemical staining of human cerebral cortex shows strong nuclear positivity in neuronal cells.
Expression
RNA: detected in 37 tissues Protein: detected in 57 cell types
RNA: detected in 37 tissues Protein: detected in 52 cell types
Retrieval
HIER pH9
HIER pH6
Antibody dilution
1:250
1:250
Literature conformity
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA000275
Antibody HPA000500
Antibody HPA000981
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 219, 112, 85, 49, 32, 25, 17.5 Lane 2: RT4 Lane 3: EFO-21 Lane 4: A-431 Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY416573)
Target mass (kDa)
38.8, 29.5, 21.2
38.9, 38.8, 29.5, 18.4
38.8, 29.5, 21.2
Antibody dilution
1:500
1:500
1:250
PROTEIN ARRAY
Antibody HPA000275
Antibody HPA000500
Antibody HPA000981
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
