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Antibody staining overlaps with antibody HPA063663.
Validated
Antibody staining overlaps with antibody HPA051239.
Standard validation
Supported
Supported
Figure description
Immunofluorescent staining of human cell line PC-3 shows localization to nucleus.
Immunofluorescent staining of human cell line RT4 shows localization to nucleus.
Antibody dilution
1:84
1:122
Literature conformity
The subcellular location is supported by literature.
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA051239
Antibody HPA063663
Antibody CAB004282
Standard validation
Approved
Figure description
Immunohistochemical staining of human small intestine shows moderate nuclear positivity in glandular cells.
Expression
RNA: detected in 36 tissues Protein: detected in 40 cell types
Retrieval
HIER pH6
Antibody dilution
1:25
Literature conformity
Partly consistent with extensive gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA051239
Antibody HPA063663
Antibody CAB004282
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Supported
Analysis performed using a standard panel of samples. Single band corresponding to the predicted size in kDa (+/-20%).
Supported
Analysis performed using a standard panel of samples. Single band corresponding to the predicted size in kDa (+/-20%).
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 220, 112, 84, 47, 32, 26, 16.8 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
57.6, 55.4, 54.7
57.6, 55.4, 54.7, 28.5, 15
57.6, 55.4, 54.7, 28.5, 15, 11.5, 4.9
Antibody dilution
1:420
1:410
1:500
PROTEIN ARRAY
Antibody HPA051239
Antibody HPA063663
Antibody CAB004282
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
