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Immunofluorescent staining of human cell line A-431 shows localization to nucleus, nucleoli fibrillar center & the Golgi apparatus.
Antibody dilution
1:26
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA038156
Antibody CAB032985
Standard validation
Approved
Approved
Figure description
Immunohistochemical staining of human prostate shows strong cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human prostate shows strong cytoplasmic positivity in glandular cells.
Expression
RNA: detected in 37 tissues Protein: detected in 39 cell types
RNA: detected in 37 tissues Protein: detected in 55 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:15
1:100
Literature conformity
No avaliable gene/protein characterization data.
No avaliable gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA038156
Antibody CAB032985
Standard validation
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY402205)
Target mass (kDa)
41.6, 37.9, 21.7
41.6, 37.9, 33.8, 29, 21.7
Antibody dilution
1:250
1:500
PROTEIN ARRAY
Antibody HPA038156
Antibody CAB032985
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
