H3F3B - Antibodies - The Human Protein Atlas

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H3F3B

TISSUE

CELL

CANCER

ANTIBODY INFORMATION

Antibody HPA042570

Antibody CAB037166

Antibody CAB037187

Antibody CAB037221

Provider

Atlas Antibodies
Sigma-Aldrich

SIGMA

Product name

HPA042570

M4819

H7162

H9286

Host species

Rabbit

Clonality

pAb

msAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Affinity

Other gene match

H3F3A - ENSG00000163041 [100%]
HIST3H3 - ENSG00000168148 [88%]
HIST2H3D - ENSG00000183598 [100%]
H3F3C - ENSG00000188375 [96%]
HIST1H3J - ENSG00000197153 [96%]
HIST1H3D - ENSG00000197409 [96%]
HIST2H3C - ENSG00000203811 [100%]
HIST2H3PS2 - ENSG00000203818 [96%]
HIST2H3A - ENSG00000203852 [100%]
HIST1H3G - ENSG00000273983 [96%]
HIST1H3B - ENSG00000274267 [96%]
HIST1H3E - ENSG00000274750 [96%]
HIST1H3I - ENSG00000275379 [96%]
HIST1H3A - ENSG00000275714 [96%]
HIST1H3F - ENSG00000277775 [96%]
HIST1H3C - ENSG00000278272 [96%]
HIST1H3H - ENSG00000278828 [96%]

Released in version

References

VALIDATION SUMMARY

Antibody HPA042570

Antibody CAB037166

Antibody CAB037187

Antibody CAB037221

Immunocytochemistry

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA042570

Antibody CAB037166

Antibody CAB037187

Antibody CAB037221

Standard validation

Supported

Figure description

Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.

Antibody dilution

1:2000

Literature conformity

The subcellular location is supported by literature.

IMMUNOHISTOCHEMISTRY

Antibody HPA042570

Antibody CAB037166

Antibody CAB037187

Antibody CAB037221

Standard validation

Supported

Figure description

Immunohistochemical staining of human vulva/anal skin shows strong nuclear positivity in epidermal cells.

Immunohistochemical staining of human testis shows strong nuclear positivity in cells in seminiferus ducts.

Immunohistochemical staining of human duodenum shows strong nuclear positivity in glandular cells.

Immunohistochemical staining of human testis shows strong nuclear positivity in cells in seminiferus ducts.

Expression

RNA: detected in 37 tissues
Protein: detected in 80 cell types

RNA: detected in 37 tissues
Protein: detected in 76 cell types

RNA: detected in 37 tissues
Protein: detected in 79 cell types

RNA: detected in 37 tissues
Protein: detected in 78 cell types

Retrieval

HIER pH6

Antibody dilution

1:30

1:24000

1:12000

1:10000

Literature conformity

Consistent with extensive gene/protein characterization data.

RNA consistency

Mainly consistent with RNA expression data.

WESTERN BLOT

Antibody HPA042570

Antibody CAB037166

Antibody CAB037187

Antibody CAB037221

Standard validation

Uncertain

Analysis performed using a standard panel of samples.
No bands detected.

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Figure description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

15.5, 15.4, 15.3, 15.2, 14.9

16.6, 15.3, 14.9, 12.8, 10.3

Antibody dilution

1:250

1:500

PROTEIN ARRAY

Antibody HPA042570

Antibody CAB037166

Antibody CAB037187

Antibody CAB037221

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:3000

ANTIGEN INFORMATION

Antibody HPA042570

Antibody CAB037166

Antibody CAB037187

Antibody CAB037221

Antigen

Recombinant protein fragment

Synthetic peptide

Length (aa)

Antigen sequence

SEAYLVGLFEDTNLCAIHAKRVTIM

Matching transcripts

H3F3B-001 - ENSP00000254810 [100%]
H3F3B-003 - ENSP00000466020 [100%]
H3F3B-005 - ENSP00000465813 [100%]
H3F3B-006 - ENSP00000465403 [100%]
H3F3B-007 - ENSP00000468714 [100%]

Other gene match

ANTIGEN VIEW

H3F3B-001	H3F3B-003	H3F3B-005	H3F3B-006	H3F3B-007

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.