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Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal and glial cells.
Immunohistochemical staining of human cerebral cortex shows positivity in neuropil.
Expression
RNA: detected in 24 tissues Protein: detected in 16 cell types
RNA: detected in 24 tissues Protein: detected in 2 cell types
Retrieval
HIER pH6
None
Antibody dilution
1:100
1:1000
Literature conformity
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
RNA consistency
Consistent with RNA expression data.
Consistent with RNA expression data.
Standard validation
Supported
Figure description
Synaptic staining in most regions of the brain, most abundant in hippocampus, cerebral cortex and olfactory bulb.
WESTERN BLOT
Antibody HPA001830
Antibody CAB000360
Standard validation
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Uncertain
Analysis performed using a standard panel of samples. No bands detected.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY418912)
Lane 1: Marker [kDa] 230, 110, 82, 49.3, 32.2, 25.5, 17.6 Lane 2: RT4 Lane 3: U-251 MG Lane 4: A-431 Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
23.3
23.3, 10.7
Antibody dilution
1:250
1:3000
PROTEIN ARRAY
Antibody HPA001830
Antibody CAB000360
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
