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Antibody staining overlaps with GFP tagged protein
Figure description
Immunofluorescent staining of transgenic HeLa cells show antibody staining in mitochondria & nucleus but excluded from the nucleoli and GFP expression in mitochondria.
Antibody dilution
1:32
Standard validation
Supported
Figure description
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & mitochondria.
Antibody dilution
1:20
Literature conformity
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA023687
Standard validation
Approved
Figure description
Immunohistochemical staining of human duodenum shows strong granular cytoplasmic positivity in glandular cells.
Expression
RNA: detected in 37 tissues Protein: detected in 63 cell types
Retrieval
HIER pH6
Antibody dilution
1:50
Literature conformity
Partly consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA023687
Standard validation
Supported
Analysis performed using a standard panel of samples. Single band corresponding to the predicted size in kDa (+/-20%).
Figure description
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
45.6, 41.7, 41, 23.4
Antibody dilution
1:250
PROTEIN ARRAY
Antibody HPA023687
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
