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CASP9

ANTIBODY INFORMATION
 
 

Antibody HPA001473

  

Antibody HPA046488

  

Antibody CAB004348

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Santa Cruz Biotechnology
  

Product name

 HPA001473   HPA046488   sc-17784  

Host species

 Rabbit   Rabbit   Mouse  

Clonality

 pAb   pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G  

Other gene match

      

Released in version

 2   13   2  

References

 1      

VALIDATION SUMMARY
 
 

Antibody HPA001473

  

Antibody HPA046488

  

Antibody CAB004348

  
Immunocytochemistry

Approved      
Immunohistochemistry

Approved   Approved   Approved  
Western blot

Supported   Uncertain   Uncertain  
Protein array

Approved   Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA001473

  

Antibody HPA046488

  

Antibody CAB004348

  

Standard validation

 Approved      

     

Figure description

 Immunofluorescent staining of human cell line A-431 shows localization to mitochondria.      

Antibody dilution

 1:4      

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.      

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA001473

  

Antibody HPA046488

  

Antibody CAB004348

  

Standard validation

 Approved   Approved   Approved  

     

Figure description

 Immunohistochemical staining of human rectum shows cytoplasmic positivity in glandular cells.   Immunohistochemical staining of human colon shows distinct cytoplasmic positivity in glandular cells.   Immunohistochemical staining of human colon shows distinct cytoplasmic positivity in glandular cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 53 cell types		   RNA: detected in 37 tissues
Protein: detected in 58 cell types		   RNA: detected in 37 tissues
Protein: detected in 61 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:25   1:150   1:600  

Literature conformity

 Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly not consistent with RNA expression data.   Mainly not consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA001473

  

Antibody HPA046488

  

Antibody CAB004348

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		  

     

Figure description

 Lane 1: Marker [kDa] 206, 113, 82, 49, 32, 26, 17.8
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: A-431
Lane 5: Liver
Lane 6: Tonsil		     Lane 1: Marker [kDa] 230, 110, 82, 49.3, 32.2, 25.5, 17.6
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 46.5, 46.3, 36.6, 28.8, 21.8   46.5, 46.3, 36.6, 30.2   46.5, 46.3, 36.6, 30.2, 28.8, 28.4, 21.8, 5.4  

Antibody dilution

 1:250   1:270   1:500  

PROTEIN ARRAY
 
 

Antibody HPA001473

  

Antibody HPA046488

  

Antibody CAB004348

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:500   1:2150    

ANTIGEN INFORMATION
 
 

Antibody HPA001473

  

Antibody HPA046488

  

Antibody CAB004348

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein  

Length (aa)

 132   107    

Antigen sequence

 
ALESLRGNADLAYILSMEPCGHCLIINNVNFCRESGLRTRTGSNIDCEKL
RRRFSSLHFMVEVKGDLTAKKMVLALLELAQQDHGALDCCVVVILSHGCQ
ASHLQFPGAVYGTDGCPVSVEKIVNIFNGTSC
  
SPEDESPGSNPEPDATPFQEGLRTFDQLDAISSLPTPSDIFVSYSTFPGF
VSWRDPKSGSWYVETLDDIFEQWAHSEDLQSLLLRVANAVSVKGIYKQMP
GCFNFLR
  

											 
																				

																		

										
											
Matching transcripts

												
																					CASP9-001 - ENSP00000330237 [100%] 
CASP9-003 - ENSP00000396540 [100%] 
CASP9-008 - ENSP00000365051 [100%] 
CASP9-009 - ENSP00000449584 [100%] 
CASP9-007 - ENSP00000408691 [86%] 

											 
																						CASP9-001 - ENSP00000330237 [100%] 
CASP9-002 - ENSP00000255256 [100%] 
CASP9-008 - ENSP00000365051 [100%] 
CASP9-009 - ENSP00000449584 [83%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
CASP9-001
CASP9-002
CASP9-003
CASP9-007
CASP9-008
CASP9-009
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
											

									
										
										
										
										
RELEVANT PUBLICATIONS

											
								

								

									 
								

								

									 
																			
											
											
											
Antibody HPA001473

												
										 
																				
											
											
											
Antibody HPA046488

												
										 
																				
											
											
											
Antibody CAB004348

												
										 
																		

																	

										
											
1.

												
																						Placental Underperfusion in a Rat Model of Intrauterine Growth Restriction Induced by a Reduced Plasma Volume Expansion

	Bibeau K et al

	PLoS One 2016;11(1):e0145982

			Application: IHC				
											 
																						
											 
																						
											 
																				

															
				
						

				 
			

		

			
	











	

		


			

				

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