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Immunohistochemical staining of human stomach shows strong cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human liver shows membranous positivity in hepatocytes.
Expression
RNA: detected in 3 tissues Protein: detected in 35 cell types
RNA: detected in 3 tissues Protein: detected in 66 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:15
1:150
Literature conformity
Partly consistent with gene/protein characterization data.
Partly consistent with gene/protein characterization data.
RNA consistency
Not consistent with RNA expression data.
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA012892
Antibody CAB014880
Standard validation
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Uncertain
Analysis performed using a standard panel of samples. Current setup is not applicable due to low RNA count.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY408009)
Target mass (kDa)
32.1
32.1, 31
Antibody dilution
1:250
1:500
PROTEIN ARRAY
Antibody HPA012892
Antibody CAB014880
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
