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Antibody staining overlaps with GFP tagged protein
Figure description
Immunofluorescent staining of transgenic HeLa cells show antibody staining in plasma membrane & cytoplasm and GFP expression in plasma membrane & cytoplasm.
Antibody dilution
1:35
Standard validation
Approved
Figure description
Immunofluorescent staining of human cell line A-431 shows no positivity.
Antibody dilution
1:34
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA014539
Antibody HPA015310
Antibody CAB002302
Standard validation
Supported
Supported
Supported
Figure description
Immunohistochemical staining of human breast shows moderate positivity in myoepithelial cells.
Immunohistochemical staining of human breast shows strong positivity in myoepithelial cells.
Immunohistochemical staining of human rectum shows distinct positivity in smooth muscle of vessels.
Expression
RNA: detected in 36 tissues Protein: detected in 39 cell types
RNA: detected in 36 tissues Protein: detected in 13 cell types
RNA: detected in 36 tissues Protein: detected in 3 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:125
1:100
1:50
Literature conformity
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
RNA consistency
Consistent with RNA expression data.
Consistent with RNA expression data.
Consistent with RNA expression data.
WESTERN BLOT
Antibody HPA014539
Antibody HPA015310
Antibody CAB002302
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Uncertain
Analysis performed using a standard panel of samples. No bands detected.
Supported
Analysis performed using a standard panel of samples. Single band corresponding to the predicted size in kDa (+/-20%).
Figure description
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
234.2, 228.1, 227.3, 224.3, 223.6
234.2, 228.1, 227.3, 224.3, 223.6
234.2, 228.1, 227.3, 224.3, 223.6
Antibody dilution
1:250
1:250
1:500
PROTEIN ARRAY
Antibody HPA014539
Antibody HPA015310
Antibody CAB002302
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
