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Antibody staining overlaps with GFP tagged protein
Figure description
Immunofluorescent staining of transgenic HeLa cells show antibody staining in cytoplasm & nucleus but excluded from the nucleoli and GFP expression in cytoplasm & nucleus but excluded from the nucleoli.
Antibody dilution
1:24
Formal validation: Independent
Validated
Antibody staining overlaps with antibody HPA031632.
Validated
Antibody staining overlaps with antibody HPA031631.
Standard validation
Supported
Supported
Figure description
Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm.
Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm.
Antibody dilution
1:24
1:25
Literature conformity
The subcellular location is supported by literature.
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA031631
Antibody HPA031632
Antibody CAB005430
Standard validation
Uncertain
Figure description
Immunohistochemical staining of human kidney shows strong cytoplasmic and membranous positivity in cells in tubules.
Expression
RNA: detected in 37 tissues Protein: detected in 15 cell types
Retrieval
HIER pH6
Antibody dilution
1:200
Literature conformity
Partly consistent with extensive gene/protein characterization data.
RNA consistency
Not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA031631
Antibody HPA031632
Antibody CAB005430
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Figure description
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
24.2, 19.7
24.2
24.2, 19.7
Antibody dilution
1:250
1:250
1:500
PROTEIN ARRAY
Antibody HPA031631
Antibody HPA031632
Antibody CAB005430
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
