HMGCS2 - Antibodies - The Human Protein Atlas

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HMGCS2

TISSUE

CELL

CANCER

ANTIBODY INFORMATION

Antibody HPA027423

Antibody HPA027442

Antibody CAB032906

Provider

Atlas Antibodies
Sigma-Aldrich

Santa Cruz Biotechnology

Product name

HPA027423

HPA027442

sc-33828

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Protein A/G

Other gene match

Released in version

References

VALIDATION SUMMARY

Antibody HPA027423

Antibody HPA027442

Antibody CAB032906

Immunocytochemistry

Supported

Immunohistochemistry

Western blot

Supported

Protein array

Supported

Approved

IMMUNOCYTOCHEMISTRY

Antibody HPA027423

Antibody HPA027442

Antibody CAB032906

Standard validation

Supported

Figure description

Immunofluorescent staining of human cell line RT4 shows localization to mitochondria.

Antibody dilution

1:109

Literature conformity

The subcellular location is supported by literature.

IMMUNOHISTOCHEMISTRY

Antibody HPA027423

Antibody HPA027442

Antibody CAB032906

Formal validation: Orthogonal

Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.

Figure description

Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.

Formal validation: Orthogonal

Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.

Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.

Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.

Figure description

Distribution of protein expression (antibody staining) and RNA expression (TPM) across 37 tissues.

Formal validation: Independent

Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.

Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.

Figure description

Distribution of protein expression (antibody staining).
Spearman correlation with HPA027442 across 72 cell types.

Distribution of protein expression (antibody staining).
Spearman correlation with HPA027423 across 72 cell types.

Standard validation

Supported

Figure description

Immunohistochemical staining of human liver shows strong cytoplasmic positivity in hepatocytes.

Immunohistochemical staining of human liver shows strong granular cytoplasmic positivity in hepatocytes.

Immunohistochemical staining of human rectum shows strong cytoplasmic positivity in glandular cells.

Expression

RNA: detected in 22 tissues
Protein: detected in 19 cell types

RNA: detected in 22 tissues
Protein: detected in 25 cell types

RNA: detected in 22 tissues
Protein: detected in 35 cell types

Retrieval

HIER pH6

Antibody dilution

1:1200

1:300

Literature conformity

Consistent with extensive gene/protein characterization data.

RNA consistency

Consistent with RNA expression data.

WESTERN BLOT

Antibody HPA027423

Antibody HPA027442

Antibody CAB032906

Standard validation

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Figure description

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

56.6, 52.5

Antibody dilution

1:250

1:500

PROTEIN ARRAY

Antibody HPA027423

Antibody HPA027442

Antibody CAB032906

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:3000

ANTIGEN INFORMATION

Antibody HPA027423

Antibody HPA027442

Antibody CAB032906

Antigen

Recombinant protein fragment

Recombinant protein

Length (aa)

Antigen sequence

ASFFSFRVSQDAAPGSPLDKLVSSTSDLPKRLASRKCVSPEEFTEIMNQR
EQFYHKVNFSPPG

ILQLTRAVQETSLTPARLLPVAHQRFSTASAVPLAKTDTWPKDVGILALE
VYFPAQYVDQTDLEKYNNVE

Matching transcripts

HMGCS2-001 - ENSP00000358414 [100%]
HMGCS2-004 - ENSP00000439495 [100%]

Other gene match

ANTIGEN VIEW

HMGCS2-001	HMGCS2-004

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.