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IL15RA

ANTIBODY INFORMATION
 
 

Antibody CAB026215

  

Provider

 R&D Systems
  

Product name

 MAB147  

Host species

 Mouse  

Clonality

 mAb  

Purity

 Protein A/G  

Other gene match

  

Released in version

 5  

References

  

VALIDATION SUMMARY
 
 

Antibody CAB026215

  
Immunocytochemistry

Uncertain  
Immunohistochemistry

Approved  
Western blot

Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody CAB026215

  

Standard validation

 Uncertain  

 

Figure description

 Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & cytosol.  

Antibody dilution

 1:19  

Literature conformity

 The subcellular location is not consistent with literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody CAB026215

  

Standard validation

 Approved  

 

Figure description

 Immunohistochemical staining of human colon shows strong cytoplasmic positivity in glandular cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 72 cell types		  

Retrieval

 HIER pH6  

Antibody dilution

 1:75  

Literature conformity

 Partly consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody CAB026215

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

 

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 37.6, 37.2, 35.4, 33.8, 33.4, 32.8, 28.2, 26.3, 24.9, 24.4, 23, 22.9, 21.1, 18.3, 18.1, 17.6, 15.7, 15.6, 15.4  

Antibody dilution

 1:500  

ANTIGEN INFORMATION
 
 

Antibody CAB026215

  

Antigen

 Recombinant protein  

Length (aa)

  

Antigen sequence

 

											 
																				

																		

										
											
Matching transcripts

												
																					
											 
																				

																		

										
											
Other gene match

												
																					
											 
																				

															
				
						

				 
			

		

			
	











	

		


			

				

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