GNL2 - Antibodies - The Human Protein Atlas

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GNL2

TISSUE

CELL

CANCER

ANTIBODY INFORMATION

Antibody HPA027163

Antibody HPA060026

Provider

Atlas Antibodies
Sigma-Aldrich

Product name

HPA027163

HPA060026

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Other gene match

Released in version

References

VALIDATION SUMMARY

Antibody HPA027163

Antibody HPA060026

Immunocytochemistry

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA027163

Antibody HPA060026

Formal validation: Genetic

Validated

Significant downregulation > 25% by both siRNA:s.

Relative fluorescence intensity (RFI)

Figure description

Nuclear region of segmented cells in 10x-images

Antibody dilution

1:76

Formal validation: Independent

Validated

Antibody staining overlaps with antibody HPA060026.

Validated

Antibody staining overlaps with antibody HPA027163.

Standard validation

Supported

Figure description

Immunofluorescent staining of human cell line A-431 shows localization to nucleoli.

Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoli.

Antibody dilution

1:100

1:62

Literature conformity

The subcellular location is supported by literature.

IMMUNOHISTOCHEMISTRY

Antibody HPA027163

Antibody HPA060026

Standard validation

Approved

Figure description

Immunohistochemical staining of human gall bladder shows distinct nucleolar positivity in glandular cells.

Expression

RNA: detected in 37 tissues
Protein: detected in 28 cell types

Retrieval

HIER pH6

Antibody dilution

1:400

Literature conformity

Consistent with gene/protein characterization data.

RNA consistency

Mainly not consistent with RNA expression data.

WESTERN BLOT

Antibody HPA027163

Antibody HPA060026

Standard validation

Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Figure description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

83.7

Antibody dilution

1:250

1:210

PROTEIN ARRAY

Antibody HPA027163

Antibody HPA060026

Standard validation

Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:3000

1:2500

ANTIGEN INFORMATION

Antibody HPA027163

Antibody HPA060026

Antigen

Recombinant protein fragment

Length (aa)

Antigen sequence

RIPFFVKPPNAEPLVAPQLLPSSSLEVVPEAAQNNPGEEVTETAGEGSES
IIKEETEENSHCDANTEMQQILTRVRQNFGKINVVPQFSGDDLVPV

NAEMSTESYDQGKDRDLVTEDTGVRNEAQEEIYKKGQSKRIWGELYKVID
SSDVVVQVLDARDPMGTRSPH

Matching transcripts

GNL2-001 - ENSP00000362153 [100%]

Other gene match

ANTIGEN VIEW

GNL2-001

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.