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Antibody staining overlaps with antibody HPA058407.
Validated
Antibody staining overlaps with antibody HPA053099.
Standard validation
Supported
Supported
Figure description
Immunofluorescent staining of human cell line MCF7 shows localization to nucleus & nuclear membrane.
Immunofluorescent staining of human cell line MCF7 shows localization to nucleus.
Antibody dilution
1:25
1:24
Literature conformity
The subcellular location is supported by literature.
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA053099
Antibody HPA058407
Standard validation
Uncertain
Uncertain
Figure description
Immunohistochemical staining of human kidney shows cytoplasmic positivity in cells in tubules.
Immunohistochemical staining of human kidney shows strong granular cytoplasmic positivity in cells in tubules.
Expression
RNA: detected in 37 tissues Protein: detected in 21 cell types
RNA: detected in 37 tissues Protein: detected in 26 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:40
1:35
Literature conformity
No avaliable gene/protein characterization data.
No avaliable gene/protein characterization data.
RNA consistency
Not consistent with RNA expression data.
Not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA053099
Antibody HPA058407
Standard validation
Supported
Analysis performed using a standard panel of samples. Single band corresponding to the predicted size in kDa (+/-20%).
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
37.5
37.5
Antibody dilution
1:120
1:80
PROTEIN ARRAY
Antibody HPA053099
Antibody HPA058407
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
