We use cookies to enhance the usability of our website. If you continue, we'll assume that you are happy to receive all cookies. More information. Don't show this again.
Immunofluorescent staining of human cell line RH-30 shows localization to nucleoplasm, cytosol & microtubule organizing center.
Antibody dilution
1:69
Literature conformity
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA054891
Antibody HPA057272
Antibody HPA070194
Standard validation
Uncertain
Uncertain
Figure description
Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells in tubules.
Immunohistochemical staining of human testis shows weak nuclear and cytoplasmic positivity in cells in seminiferus ducts.
Expression
RNA: detected in 34 tissues Protein: detected in 51 cell types
RNA: detected in 34 tissues Protein: detected in 31 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:60
1:1500
Literature conformity
Partly consistent with gene/protein characterization data.
Partly consistent with gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
Not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA054891
Antibody HPA057272
Antibody HPA070194
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
98.7, 96.7
98.7, 96.7
98.7
Antibody dilution
1:230
1:1040
1:230
PROTEIN ARRAY
Antibody HPA054891
Antibody HPA057272
Antibody HPA070194
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.