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Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & cytosol.
Antibody dilution
1:200
Literature conformity
The subcellular location is not consistent with literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA030170
Antibody HPA030171
Antibody HPA030172
Antibody HPA030173
Formal validation: Independent
Validated
Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.
Validated
Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.
Validated
Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.
Validated
Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.
Figure description
Distribution of protein expression (antibody staining). Pearson correlation with HPA030171 across 46 cell lines.
Distribution of protein expression (antibody staining). Pearson correlation with HPA030172 across 41 cell lines. Pearson correlation with HPA030170 across 46 cell lines.
Distribution of protein expression (antibody staining). Pearson correlation with HPA030173 across 41 cell lines. Pearson correlation with HPA030171 across 41 cell lines.
Distribution of protein expression (antibody staining). Pearson correlation with HPA030172 across 41 cell lines.
Standard validation
Supported
Supported
Supported
Supported
Figure description
Immunohistochemical staining of human placenta shows strong membranous and nuclear positivity in trophoblastic cells.
Immunohistochemical staining of human placenta shows strong cytoplasmic and nuclear membranous positivity in decidual cells.
Immunohistochemical staining of human duodenum shows moderate granular cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human liver shows strong cytoplasmic positivity in hepatocytes.
Expression
RNA: detected in 33 tissues Protein: detected in 63 cell types
RNA: detected in 33 tissues Protein: detected in 79 cell types
RNA: detected in 33 tissues Protein: detected in 78 cell types
RNA: detected in 33 tissues Protein: detected in 73 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:350
1:800
1:1400
1:2000
Literature conformity
Partly consistent with gene/protein characterization data.
Partly consistent with gene/protein characterization data.
Partly consistent with gene/protein characterization data.
Partly consistent with gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA030170
Antibody HPA030171
Antibody HPA030172
Antibody HPA030173
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Figure description
Target mass (kDa)
161.9, 153.1
161.9, 153.1
161.9, 153.1
161.9, 153.1
Antibody dilution
1:250
1:250
1:250
1:250
PROTEIN ARRAY
Antibody HPA030170
Antibody HPA030171
Antibody HPA030172
Antibody HPA030173
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
