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LAMC1

ANTIBODY INFORMATION
 
 

Antibody HPA001908

  

Antibody HPA001909

  

Antibody CAB004486

  

Antibody CAB078163

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Santa Cruz Biotechnology
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA001908   HPA001909   sc-13144   AMAb91138  

Host species

 Rabbit   Rabbit   Mouse   Mouse  

Clonality

 pAb   pAb   mAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G   Protein A/G  

Other gene match

        

Released in version

 2   2   2   16  

References

 2   1      

VALIDATION SUMMARY
 
 

Antibody HPA001908

  

Antibody HPA001909

  

Antibody CAB004486

  

Antibody CAB078163

  
Immunocytochemistry

  Uncertain      
Immunohistochemistry


Validated

Validated
  

Validated

Validated
   Supported   Supported  
Western blot

Uncertain   Supported   Supported   Supported  
Protein array

Supported   Supported      

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA001908

  

Antibody HPA001909

  

Antibody CAB004486

  

Antibody CAB078163

  

Standard validation

   Uncertain      

       

Figure description

   Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & endoplasmic reticulum.      

Antibody dilution

   1:74      

Literature conformity

   The subcellular location is not consistent with literature.      

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA001908

  

Antibody HPA001909

  

Antibody CAB004486

  

Antibody CAB078163

  

Formal validation: Orthogonal
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		      

 

 

  
 

 

      

Figure description

 Distribution of protein expression (antibody staining) and RNA expression (TPM) across 43 cell lines.   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 43 cell lines.      
 

Formal validation: Independent
Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.		  
Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.		      

 

  
 

      

Figure description

 Distribution of protein expression (antibody staining).
Pearson correlation with HPA001909 across 45 cell lines.		   Distribution of protein expression (antibody staining).
Pearson correlation with HPA001908 across 45 cell lines.		      
 

Standard validation

 Supported   Supported   Supported   Supported  

       

Figure description

 Immunohistochemical staining of human placenta shows strong cytoplasmic and membranous positivity in trophoblastic cells.   Immunohistochemical staining of human placenta shows strong cytoplasmic and membranous positivity in trophoblastic cells.   Immunohistochemical staining of human heart muscle shows strong membranous positivity in myocytes.   Immunohistochemical staining of human placenta shows strong positivity in basal membranes.  

Expression

 RNA: detected in 36 tissues
Protein: detected in 51 cell types		   RNA: detected in 36 tissues
Protein: detected in 39 cell types		   RNA: detected in 36 tissues
Protein: detected in 32 cell types		   RNA: detected in 36 tissues
Protein: detected in 3 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:500   1:400   1:200   1:5000  

Literature conformity

 Partly consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA001908

  

Antibody HPA001909

  

Antibody CAB004486

  

Antibody CAB078163

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

       

Figure description

   Lane 1: Marker [kDa] 206, 113, 82, 49, 32, 26, 17.8
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: A-431
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 230, 110, 82, 49.3, 32.2, 25.5, 17.6
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: A-431
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 460, 268, 238, 171, 117, 71, 55, 41, 31
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 177.6   177.6   177.6, 4.6   177.6, 4.6  

Antibody dilution

 1:250   1:250   1:500   1:500  

PROTEIN ARRAY
 
 

Antibody HPA001908

  

Antibody HPA001909

  

Antibody CAB004486

  

Antibody CAB078163

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		      

       

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.      

Antibody dilution

 1:3000   1:3000      

ANTIGEN INFORMATION
 
 

Antibody HPA001908

  

Antibody HPA001909

  

Antibody CAB004486

  

Antibody CAB078163

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein   Recombinant protein  

Length (aa)

 149   111      

Antigen sequence

 
ECREGFVGNRCDQCEENYFYNRSWPGCQECPACYRLVKDKVADHRVKLQE
LESLIANLGTGDEMVTDQAFEDRLKEAEREVMDLLREAQDVKDVDQNLMD
RLQRVNNTLSSQISRLQNIRNTIEETGNLAEQARAHVENTERLIEIASR
  
STKAEAERTFAEVTDLDNEVNNMLKQLQEAEKELKRKQDDADQDMMMAGM
ASQAAQEAEINARKAKNSVTSLLSIINDLLEQLGQLDTVDLNKLNEIEGT
LNKAKDEMKVS
  

											 
																						

											 
																				

																		

										
											
Matching transcripts

												
																					LAMC1-001 - ENSP00000258341 [100%] 

											 
																						LAMC1-001 - ENSP00000258341 [100%] 

											 
																						
											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
LAMC1-001
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
											

									
										
										
										
										
RELEVANT PUBLICATIONS

											
								

								

									 
								

								

									 
																			
											
											
											
Antibody HPA001908

												
										 
																				
											
											
											
Antibody HPA001909

												
										 
																				
											
											
											
Antibody CAB004486

												
										 
																				
											
											
											
Antibody CAB078163

												
										 
																		

																	

										
											
1.

												
																						Systematic validation of antibody binding and protein subcellular localization using siRNA and confocal microscopy

	Stadler C et al

	J Proteomics 2012;75(7):2236-51

			
											 
																							The diagnostic and biological implications of laminin expression in serous tubal intraepithelial carcinoma

	Kuhn E et al

	Am J Surg Pathol 2012;36(12):1826-34

			
											 
																						
											 
																						
											 
																				

																		

										
											
2.

												
																						Laminin C1 expression by uterine carcinoma cells is associated with tumor progression

	Kashima H et al

	Gynecol Oncol 2015;139(2):338-44

			Application: IHC				
											 
																						
											 
																						
											 
																						
											 
																				

															
				
						

				 
			

		

			
	











	

		


			

				

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by the Knut & Alice Wallenberg foundation.