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KIAA1033

ANTIBODY INFORMATION
 
 

Antibody HPA045666

  

Antibody HPA046737

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA045666   HPA046737  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 12   12  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA045666

  

Antibody HPA046737

  
Immunocytochemistry


Validated
  

Validated
  
Immunohistochemistry

Uncertain   Uncertain  
Western blot

Uncertain   Uncertain  
Protein array

Supported   Approved  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA045666

  

Antibody HPA046737

  

Formal validation: Independent
Validated

Antibody staining overlaps with antibody HPA046737.		  
Validated

Antibody staining overlaps with antibody HPA045666.		  
 

Standard validation

 Approved   Approved  

   

Figure description

 Immunofluorescent staining of human cell line Hep G2 shows localization to nucleoplasm.   Immunofluorescent staining of human cell line HeLa shows localization to nucleoplasm.  

Antibody dilution

 1:9   1:9  

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.   The subcellular location is partly supported by literature or no literature is available.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA045666

  

Antibody HPA046737

  

Standard validation

 Uncertain   Uncertain  

   

Figure description

 Immunohistochemical staining of human placenta shows nuclear positivity in trophoblastic cells.   Immunohistochemical staining of human placenta shows cytoplasmic and membranous positivity in trophoblastic cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 76 cell types		   RNA: detected in 37 tissues
Protein: detected in 59 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:600   1:100  

Literature conformity

 Not consistent with gene/protein characterization data.   Not consistent with gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA045666

  

Antibody HPA046737

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		   Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		  

   

Figure description

    

Target mass (kDa)

 136.5, 136.4   136.5, 136.4  

Antibody dilution

 1:250   1:90  

PROTEIN ARRAY
 
 

Antibody HPA045666

  

Antibody HPA046737

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:500   1:700  

ANTIGEN INFORMATION
 
 

Antibody HPA045666

  

Antibody HPA046737

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 95   93  

Antigen sequence

 
KVLNKVITVYAALCCEIKKLKYEAETKFYNGLLFYGEGATDASMVEGDCQ
IQMGRFISFLQELSCFVTRCYEVVMNVVHQLAALYISNKIAPKII
  
SVHTHLKLDDRNPFKVGMKDLALFFSLNPIRFFNRFIDIRAYVTHYLDKT
FYNLTTVALHDWATYSEMRNLATQRYGLVMTEAHLPSQTLEQG
 

Matching transcripts

 KIAA1033-001 - ENSP00000328062 [100%]
KIAA1033-201 - ENSP00000484713 [100%]
  KIAA1033-001 - ENSP00000328062 [100%]
KIAA1033-201 - ENSP00000484713 [100%]
 

Other gene match

    
ANTIGEN VIEW
KIAA1033-001
KIAA1033-201
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.