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EPRS

ANTIBODY INFORMATION
 
 

Antibody HPA026490

  

Antibody HPA030052

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA026490   HPA030052  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 5   6  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA026490

  

Antibody HPA030052

  
Immunocytochemistry

 

Validated
  
Immunohistochemistry

Supported   Supported  
Western blot

Supported   Supported  
Protein array

Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA026490

  

Antibody HPA030052

  

Formal validation: Genetic

  
Validated

Significant downregulation > 25% by both siRNA:s.		  

 
 

Relative fluorescence intensity (RFI)

  

Figure description

   Cytoplasmic region of segmented cells in 10x-images  

Antibody dilution

   1:48  
 

Standard validation

   Approved  

   

Figure description

   Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.  

Antibody dilution

   1:50  

Literature conformity

   The subcellular location is partly supported by literature or no literature is available.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA026490

  

Antibody HPA030052

  

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human smooth muscle shows strong cytoplasmic positivity in smooth muscle cells.   Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 53 cell types		   RNA: detected in 37 tissues
Protein: detected in 73 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:200   1:75  

Literature conformity

 No avaliable gene/protein characterization data.   No avaliable gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA026490

  

Antibody HPA030052

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		  

   

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 170.6   170.6  

Antibody dilution

 1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA026490

  

Antibody HPA030052

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA026490

  

Antibody HPA030052

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 105   88  

Antigen sequence

 
CVQVVIIPCGITNALSEEDKEALIAKCNDYRRRLLSVNIRVRADLRDNYS
PGWKFNHWELKGVPIRLEVGPRDMKSCQFVAVRRDTGEKLTVAENEAETK
LQAIL
  
DWYSQVITKSEMIEYHDISGCYILRPWAYAIWEAIKDFFDAEIKKLGVEN
CYFPMFVSQSALEKEKTHVADFAPEVAWVTRSGKTELA
 

Matching transcripts

 EPRS-001 - ENSP00000355890 [100%]
  EPRS-001 - ENSP00000355890 [100%]
 

Other gene match

    
ANTIGEN VIEW
EPRS-001
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.