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CCL21

ANTIBODY INFORMATION
 
 

Antibody HPA051210

  

Antibody CAB005067

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Santa Cruz Biotechnology
  

Product name

 HPA051210   sc-5808  

Host species

 Rabbit   Goat  

Clonality

 pAb   msAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity  

Other gene match

    

Released in version

 13   2  

References

 2    

VALIDATION SUMMARY
 
 

Antibody HPA051210

  

Antibody CAB005067

  
Immunohistochemistry

Supported   Supported  
Western blot

Supported   Uncertain  
Protein array

Supported    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA051210

  

Antibody CAB005067

  

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human lymph node shows distinct positivity in a subset of lymphoid cells.   Immunohistochemical staining of human lymph node shows positivity in a small subset of vessels.  

Expression

 RNA: detected in 35 tissues
Protein: detected in 12 cell types		   RNA: detected in 35 tissues
Protein: detected in 16 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:500   1:20  

Literature conformity

 Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA051210

  

Antibody CAB005067

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		  

   

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		    

Target mass (kDa)

 14.6, 13.6   14.6, 13.6  

Antibody dilution

 1:210   1:500  

PROTEIN ARRAY
 
 

Antibody HPA051210

  

Antibody CAB005067

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:1650    

ANTIGEN INFORMATION
 
 

Antibody HPA051210

  

Antibody CAB005067

  

Antigen

 Recombinant protein fragment   Synthetic peptide  

Length (aa)

 52    

Antigen sequence

 
KELWVQQLMQHLDKTPSPQKPAQGCRKDRGASKTGKKGKGSKGCKRTERS
QT
  

											 
																				

																		

										
											
Matching transcripts

												
																					CCL21-002 - ENSP00000259607 [100%] 
CCL21-001 - ENSP00000368069 [88%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
CCL21-001
CCL21-002
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
											

									
										
										
										
										
RELEVANT PUBLICATIONS

											
								

								

									 
								

								

									 
																			
											
											
											
Antibody HPA051210

												
										 
																				
											
											
											
Antibody CAB005067

												
										 
																		

																	

										
											
1.

												
																						The transcriptomic and proteomic landscapes of bone marrow and secondary lymphoid tissues

	Andersson S et al

	PLoS One 2014;9(12):e115911

			
											 
																						
											 
																				

																		

										
											
2.

												
																						Expression of CCL21 in Ewing sarcoma shows an inverse correlation with metastases and is a candidate target for immunotherapy

	Sand LG et al

	Cancer Immunol Immunother 2016;65(8):995-1002

			
											 
																						
											 
																				

															
				
						

				 
			

		

			
	











	

		


			

				

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