We use cookies to enhance the usability of our website. If you continue, we'll assume that you are happy to receive all cookies. More information. Don't show this again.
Antibody staining overlaps with antibody HPA029980.
Validated
Antibody staining overlaps with antibody HPA029979.
Standard validation
Supported
Supported
Supported
Figure description
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.
Antibody dilution
1:20
1:100
1:200
Literature conformity
The subcellular location is supported by literature.
The subcellular location is supported by literature.
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA029954
Antibody HPA029979
Antibody HPA029980
Formal validation: Independent
Validated
Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.
Validated
Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.
Validated
Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.
Figure description
Distribution of protein expression (antibody staining). Pearson correlation with HPA029980 across 45 cell lines. Pearson correlation with HPA029979 across 45 cell lines.
Distribution of protein expression (antibody staining). Pearson correlation with HPA029980 across 45 cell lines. Pearson correlation with HPA029954 across 45 cell lines.
Distribution of protein expression (antibody staining). Pearson correlation with HPA029979 across 45 cell lines. Pearson correlation with HPA029954 across 45 cell lines.
Formal validation: Independent
Validated
Spearman correlation >0.6 for protein expression in tissues using independent antibodies.
Validated
Spearman correlation >0.6 for protein expression in tissues using independent antibodies.
Figure description
Distribution of protein expression (antibody staining). Spearman correlation with HPA029980 across 72 cell types.
Distribution of protein expression (antibody staining). Spearman correlation with HPA029954 across 72 cell types.
Standard validation
Supported
Supported
Supported
Figure description
Immunohistochemical staining of human testis shows strong nuclear positivity in cells in seminiferus ducts and leydig cells.
Immunohistochemical staining of human placenta shows strong nucleolar positivity in trophoblastic cells.
Immunohistochemical staining of human testis shows strong nuclear positivity in cells in seminiferus ducts and leydig cells.
Expression
RNA: detected in 37 tissues Protein: detected in 58 cell types
RNA: detected in 37 tissues Protein: detected in 58 cell types
RNA: detected in 37 tissues Protein: detected in 66 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:70
1:225
1:375
Literature conformity
Consistent with gene/protein characterization data.
Consistent with gene/protein characterization data.
Consistent with gene/protein characterization data.
RNA consistency
Consistent with RNA expression data.
Consistent with RNA expression data.
Consistent with RNA expression data.
WESTERN BLOT
Antibody HPA029954
Antibody HPA029979
Antibody HPA029980
Standard validation
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY414851)
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
95.3, 41.6
95.3, 41.6
95.3
Antibody dilution
1:250
1:250
1:250
PROTEIN ARRAY
Antibody HPA029954
Antibody HPA029979
Antibody HPA029980
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.