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Immunohistochemical staining of human tonsil shows strong nuclear positivity in lymphoid cells outside reaction centra.
Immunohistochemical staining of human tonsil shows distinct nuclear positivity in subsets of lymphoid cells.
Immunohistochemical staining of human lymph node shows strong nuclear positivity in lymphoid cells outside reaction centra.
Expression
RNA: detected in 23 tissues Protein: detected in 27 cell types
RNA: detected in 23 tissues Protein: detected in 6 cell types
RNA: detected in 23 tissues Protein: detected in 36 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:250
1:100
1:65
Literature conformity
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA002038
Antibody HPA002698
Antibody CAB013508
Standard validation
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY419304)
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY419304)
Target mass (kDa)
51.8
51.8
51.8
Antibody dilution
1:250
1:250
1:500
PROTEIN ARRAY
Antibody HPA002038
Antibody HPA002698
Antibody CAB013508
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
