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Antibody staining overlaps with antibody HPA062198.
Validated
Antibody staining overlaps with antibody HPA040763.
Standard validation
Supported
Supported
Figure description
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.
Antibody dilution
1:90
1:6
Literature conformity
The subcellular location is supported by literature.
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA040763
Antibody HPA062198
Standard validation
Approved
Figure description
Immunohistochemical staining of human esophagus shows strong cytoplasmic positivity in squamous epithelial cells.
Expression
RNA: detected in 37 tissues Protein: detected in 45 cell types
Retrieval
HIER pH6
Antibody dilution
1:400
Literature conformity
No avaliable gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA040763
Antibody HPA062198
Standard validation
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
53, 45.4, 18.3, 13.3, 10.4
53
Antibody dilution
1:250
1:80
PROTEIN ARRAY
Antibody HPA040763
Antibody HPA062198
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
