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MAPKBP1

ANTIBODY INFORMATION
 
 

Antibody HPA030832

  

Antibody HPA030833

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA030832   HPA030833  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 6   13  

References

 1    

VALIDATION SUMMARY
 
 

Antibody HPA030832

  

Antibody HPA030833

  
Immunocytochemistry

  Approved  
Immunohistochemistry

Approved   Approved  
Western blot

Uncertain   Uncertain  
Protein array

Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA030832

  

Antibody HPA030833

  

Standard validation

   Approved  

   

Figure description

   Immunofluorescent staining of human cell line U-2 OS shows localization to nucleus & nucleoli.  

Antibody dilution

   1:84  

Literature conformity

   The subcellular location is partly supported by literature or no literature is available.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA030832

  

Antibody HPA030833

  

Standard validation

 Approved   Approved  

   

Figure description

 Immunohistochemical staining of human testis shows distinct cytoplasmic positivity in cells in seminiferous ducts.   Immunohistochemical staining of human testis shows distinct cytoplasmic positivity in cells in seminiferous ducts.  

Expression

 RNA: detected in 35 tissues
Protein: detected in 67 cell types		   RNA: detected in 35 tissues
Protein: detected in 76 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:100   1:1200  

Literature conformity

 No avaliable gene/protein characterization data.   No avaliable gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA030832

  

Antibody HPA030833

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		   Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		  

   

Figure description

    

Target mass (kDa)

 163.8, 163.1, 134.3   163.8, 163.1, 134.3  

Antibody dilution

 1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA030832

  

Antibody HPA030833

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA030832

  

Antibody HPA030833

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 87   77  

Antigen sequence

 
EKHSPDSACSVDYSSSCLSSPEHPTEDSESTEPLSVDGISSDLEEPAEGD
EEEEEEEGGMGPYGLQEGSPQTPDQEQFLKQHFETLA
  
PSSPQRASGPNRHQAPSMLSPGPALSSDSDKEGEDEGTEEELPALPVLAK
STKKALASVPSPALPRSLSHWEMSRAQ
 

Matching transcripts

 MAPKBP1-001 - ENSP00000397570 [100%]
MAPKBP1-002 - ENSP00000393099 [100%]
MAPKBP1-006 - ENSP00000426154 [100%]
  MAPKBP1-001 - ENSP00000397570 [100%]
MAPKBP1-002 - ENSP00000393099 [100%]
MAPKBP1-006 - ENSP00000426154 [100%]
 

Other gene match

    
ANTIGEN VIEW
MAPKBP1-001
MAPKBP1-002
MAPKBP1-006

RELEVANT PUBLICATIONS
 
 

Antibody HPA030832

  

Antibody HPA030833

  

1.

 The c-Jun N-terminal kinase (JNK)-binding protein (JNKBP1) acts as a negative regulator of NOD2 protein signaling by inhibiting its oligomerization process
Lecat A et al
J Biol Chem 2012;287(35):29213-26
    
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.