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CASC5

ANTIBODY INFORMATION
 
 

Antibody HPA026624

  

Antibody HPA049647

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA026624   HPA049647  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 6   12  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA026624

  

Antibody HPA049647

  
Immunocytochemistry

Supported   Supported  
Immunohistochemistry


Validated
    
Western blot

Uncertain   Uncertain  
Protein array

Approved   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA026624

  

Antibody HPA049647

  

Standard validation

 Supported   Supported  

   

Figure description

 Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.   Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm, nuclear bodies & cytosol.  

Antibody dilution

 1:50   1:81  

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA026624

  

Antibody HPA049647

  

Formal validation: Orthogonal
Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.		    

 

 

    

Figure description

 Distribution of protein expression (antibody staining) and RNA expression (TPM) across 37 tissues.    
 

Standard validation

 Supported    

   

Figure description

 Immunohistochemical staining of human testis shows distinct nuclear positivity in cells in seminiferus ducts.    

Expression

 RNA: detected in 13 tissues
Protein: detected in 28 cell types		    

Retrieval

 HIER pH6    

Antibody dilution

 1:60    

Literature conformity

 Consistent with extensive gene/protein characterization data.    

RNA consistency

 Mainly consistent with RNA expression data.    

WESTERN BLOT
 
 

Antibody HPA026624

  

Antibody HPA049647

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		  

   

Figure description

    

Target mass (kDa)

 265.4, 262.6   265.4, 262.6, 195.6  

Antibody dilution

 1:250   1:270  

PROTEIN ARRAY
 
 

Antibody HPA026624

  

Antibody HPA049647

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:3250  

ANTIGEN INFORMATION
 
 

Antibody HPA026624

  

Antibody HPA049647

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 149   147  

Antigen sequence

 
HHTEEDIDKSANSVLIKNLSRTPSSCSSSLDSIKADGTSLDFSTYRSSQM
ESQFLRDTICEESLREKLQDGRITIREFFILLQVHILIQKPRQSNLPGNF
TVNTPPTPEDLMLSQYVYRPKIQIYREDCEARRQKIEELKLSASNQDKL
  
DVTKQVIQTHVNAGEAPDPVITSNVPCFHSIKPNLNNLNGKTGEFLAFQT
VHLPPLPEQLLELGNKAHNDMHIVQATEIHNINIISSNAKDSRDEENKKS
HNGAETTSLPPKTVFKDKVRRCSLGIFLPRLPNKRNCSVTGIDDLEQ
 

Matching transcripts

 CASC5-001 - ENSP00000382576 [100%]
CASC5-002 - ENSP00000335463 [100%]
  CASC5-001 - ENSP00000382576 [100%]
CASC5-002 - ENSP00000335463 [100%]
CASC5-201 - ENSP00000432654 [100%]
 

Other gene match

    
ANTIGEN VIEW
CASC5-001
CASC5-002
CASC5-201
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.