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SLC28A2

ANTIBODY INFORMATION
 
 

Antibody HPA046068

  

Antibody HPA055623

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA046068   HPA055623  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 12   13  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA046068

  

Antibody HPA055623

  
Immunohistochemistry


Validated
   Supported  
Western blot

Uncertain   Uncertain  
Protein array

Supported   Supported  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA046068

  

Antibody HPA055623

  

Formal validation: Orthogonal
Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.		    

 

 

    

Figure description

 Distribution of protein expression (antibody staining) and RNA expression (TPM) across 37 tissues.    
 

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human small intestine shows strong luminal membranous positivity in glandular cells.   Immunohistochemical staining of human duodenum shows strong luminal membrane positivity in glandular cells.  

Expression

 RNA: detected in 10 tissues
Protein: detected in 7 cell types		   RNA: detected in 10 tissues
Protein: detected in 12 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:2000   1:1000  

Literature conformity

 Partly consistent with gene/protein characterization data.   Partly consistent with gene/protein characterization data.  

RNA consistency

 Consistent with RNA expression data.   Consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA046068

  

Antibody HPA055623

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		   Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		  

   

Figure description

    

Target mass (kDa)

 71.9   71.9  

Antibody dilution

 1:250   1:450  

PROTEIN ARRAY
 
 

Antibody HPA046068

  

Antibody HPA055623

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:3600  

ANTIGEN INFORMATION
 
 

Antibody HPA046068

  

Antibody HPA055623

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 79   69  

Antigen sequence

 
MEKASGRQSIALSTVETGTVNLGLELMEKEVEPEGSKRTDAQGHSLGDGL
GPSTYQRRSRWPFSKARSFCKTHASLFKK
  
GAEADCVSFPNTSFTNRTYETYMCCRGLFQSTSLNGTNPPSFSGPWEDKE
FSAMALTNCCGFYNNTVCA
 

Matching transcripts

 SLC28A2-001 - ENSP00000315006 [99%]
  SLC28A2-001 - ENSP00000315006 [100%]
 

Other gene match

    
ANTIGEN VIEW
SLC28A2-001
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.