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Immunofluorescent staining of human cell line A-431 shows localization to mitochondria.
Antibody dilution
1:100
Literature conformity
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA026481
Antibody HPA026485
Antibody HPA026533
Formal validation: Independent
Validated
Spearman correlation >0.6 for protein expression in tissues using independent antibodies.
Validated
Spearman correlation >0.6 for protein expression in tissues using independent antibodies.
Figure description
Distribution of protein expression (antibody staining). Spearman correlation with HPA026533 across 75 cell types.
Distribution of protein expression (antibody staining). Spearman correlation with HPA026485 across 75 cell types.
Standard validation
Supported
Supported
Supported
Figure description
Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in distal tubules.
Immunohistochemical staining of human rectum shows strong granular positivity in glandular cells.
Immunohistochemical staining of human testis shows strong cytoplasmic positivity in leydig cells.
Expression
RNA: detected in 37 tissues Protein: detected in 50 cell types
RNA: detected in 37 tissues Protein: detected in 58 cell types
RNA: detected in 37 tissues Protein: detected in 57 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:20
1:600
1:50
Literature conformity
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA026481
Antibody HPA026485
Antibody HPA026533
Standard validation
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY419653)
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY419653)
Target mass (kDa)
53.5
53.5, 36.2
53.5, 36.2
Antibody dilution
1:250
1:250
1:250
PROTEIN ARRAY
Antibody HPA026481
Antibody HPA026485
Antibody HPA026533
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody dilution
1:3000
1:3000
1:3000
ANTIGEN INFORMATION
Antibody HPA026481
Antibody HPA026485
Antibody HPA026533
Antigen
Recombinant protein fragment
Recombinant protein fragment
Recombinant protein fragment
Length (aa)
103
82
75
Antigen sequence
LQFPILNASVDENCQNITYKASHNIGIAMDTEQGLIVPNVKNVQICSIFD
IATELNRLQKLGSVGQLSTTDLTGGTFTLSNIGSIGGTFAKPVIMPPEVA
IGA
