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RTCA

ANTIBODY INFORMATION
 
 

Antibody HPA027982

  

Antibody HPA027990

  

Antibody HPA028151

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA027982   HPA027990   HPA028151  

Host species

 Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

      

Released in version

 6   13   6  

References

 1      

VALIDATION SUMMARY
 
 

Antibody HPA027982

  

Antibody HPA027990

  

Antibody HPA028151

  
Immunocytochemistry


Validated
  

Validated
    
Immunohistochemistry

Uncertain     Uncertain  
Western blot

Supported   Supported   Uncertain  
Protein array

Supported   Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA027982

  

Antibody HPA027990

  

Antibody HPA028151

  

Formal validation: Independent
Validated

Antibody staining overlaps with antibody HPA027990.		  
Validated

Antibody staining overlaps with antibody HPA027982.		    
 

Standard validation

 Uncertain   Supported    

     

Figure description

 Immunofluorescent staining of human cell line A-431 shows localization to nucleus.   Immunofluorescent staining of human cell line Hep G2 shows localization to nucleoplasm.    

Antibody dilution

 1:20   1:53    

Literature conformity

 The subcellular location is not consistent with literature.   The subcellular location is supported by literature.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA027982

  

Antibody HPA027990

  

Antibody HPA028151

  

Standard validation

 Uncertain     Uncertain  

     

Figure description

 Immunohistochemical staining of human kidney shows moderate nuclear and cytoplasmic positivity in cells in tubules.     Immunohistochemical staining of human testis shows strong cytoplasmic positivity in leydig cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 42 cell types		     RNA: detected in 37 tissues
Protein: detected in 57 cell types		  

Retrieval

 HIER pH6     HIER pH6  

Antibody dilution

 1:60     1:50  

Literature conformity

 Partly consistent with extensive gene/protein characterization data.     Not consistent with gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.     Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA027982

  

Antibody HPA027990

  

Antibody HPA028151

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		  

     

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		    

Target mass (kDa)

 40.7, 39.3   40.7, 39.3   40.7, 39.3  

Antibody dilution

 1:250   1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA027982

  

Antibody HPA027990

  

Antibody HPA028151

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:3000   1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA027982

  

Antibody HPA027990

  

Antibody HPA028151

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 83   86   78  

Antigen sequence

 
LFAASPSELHLKGGTNAEMAPQIDYTVMVFKPIVEKFGFIFNCDIKTRGY
YPKGGGEVIVRMSPVKQLNPINLTERGCVTKIY
  
LLANLRHGGTVDEYLQDQLIVFMALANGVSRIKTGPVTLHTQTAIHFAEQ
IAKAKFIVKKSEDEEDAAKDTYIIECQGIGMTNPNL
  
PLRVQKIRAGRSTPGLRPQHLSGLEMIRDLCDGQLEGAEIGSTEITFTPE
KIKGGIHTADTKTAGSVCLLMQVSMPCV
 

Matching transcripts

 RTCA-001 - ENSP00000359146 [100%]
RTCA-002 - ENSP00000260563 [100%]
  RTCA-001 - ENSP00000359146 [100%]
RTCA-002 - ENSP00000260563 [100%]
  RTCA-001 - ENSP00000359146 [100%]
RTCA-002 - ENSP00000260563 [86%]
 

Other gene match

      
ANTIGEN VIEW
RTCA-001
RTCA-002

RELEVANT PUBLICATIONS
 
 

Antibody HPA027982

  

Antibody HPA027990

  

Antibody HPA028151

  

1.

 Regulation of axon regeneration by the RNA repair and splicing pathway
Song Y et al
Nat Neurosci 2015;18(6):817-25
      
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.