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HADHB

ANTIBODY INFORMATION
 
 

Antibody HPA037539

  

Antibody HPA037540

  

Antibody HPA066099

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA037539   HPA037540   HPA066099  

Host species

 Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

      

Released in version

 7   13   14  

References

      

VALIDATION SUMMARY
 
 

Antibody HPA037539

  

Antibody HPA037540

  

Antibody HPA066099

  
Immunocytochemistry

    Supported  
Immunohistochemistry

Supported   Supported    
Western blot

Supported   Supported   Supported  
Protein array

Approved   Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA037539

  

Antibody HPA037540

  

Antibody HPA066099

  

Standard validation

     Supported  

     

Figure description

     Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.  

Antibody dilution

     1:141  

Literature conformity

     The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA037539

  

Antibody HPA037540

  

Antibody HPA066099

  

Standard validation

 Supported   Supported    

     

Figure description

 Immunohistochemical staining of human heart muscle shows strong cytoplasmic positivity in myocytes.   Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells in tubules.    

Expression

 RNA: detected in 37 tissues
Protein: detected in 79 cell types		   RNA: detected in 37 tissues
Protein: detected in 64 cell types		    

Retrieval

 HIER pH6   HIER pH6    

Antibody dilution

 1:750   1:100    

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.    

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.    

WESTERN BLOT
 
 

Antibody HPA037539

  

Antibody HPA037540

  

Antibody HPA066099

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

     

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 51.3, 49.6, 48.9, 37.9   51.3, 49.6, 48.9   51.3, 49.6, 37.9, 18.8, 16.1, 9.4  

Antibody dilution

 1:250   1:250   1:470  

PROTEIN ARRAY
 
 

Antibody HPA037539

  

Antibody HPA037540

  

Antibody HPA066099

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:3000   1:5650  

ANTIGEN INFORMATION
 
 

Antibody HPA037539

  

Antibody HPA037540

  

Antibody HPA066099

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 91   92   91  

Antigen sequence

 
LRDFMYVSQDPKDQLLLGPTYATPKVLEKAGLTMNDIDAFEFHEAFSGQI
LANFKAMDSDWFAENYMGRKTKVGLPPLEKFNNWGGSLSLG
  
GTVIQEVKTSNVAREAALGAGFSDKTPAHTVTMACISANQAMTTGVGLIA
SGQCDVIVAGGVELMSDVPIRHSRKMRKLMLDLNKAKSMGQR
  
LPTASKWALRFSIRPLSCSSQLRAAPAVQTKTKKTLAKPNIRNVVVVDGV
RTPFLLSGTSYKDLMPHDLARAALTGLLHRTSVPKEVVDYI
 

Matching transcripts

 HADHB-001 - ENSP00000325136 [100%]
HADHB-002 - ENSP00000385411 [100%]
HADHB-201 - ENSP00000444295 [100%]
HADHB-202 - ENSP00000442665 [100%]
  HADHB-001 - ENSP00000325136 [100%]
HADHB-201 - ENSP00000444295 [100%]
HADHB-202 - ENSP00000442665 [100%]
  HADHB-001 - ENSP00000325136 [100%]
HADHB-002 - ENSP00000385411 [100%]
HADHB-004 - ENSP00000404633 [100%]
HADHB-007 - ENSP00000415300 [100%]
HADHB-201 - ENSP00000444295 [84%]
HADHB-005 - ENSP00000413103 [82%]
 

Other gene match

      
ANTIGEN VIEW
HADHB-001
HADHB-002
HADHB-004
HADHB-005
HADHB-007
HADHB-201
HADHB-202
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.