Cell atlas - LRPPRC - The Human Protein Atlas

GENERAL INFORMATION
Gene name	LRPPRC
Gene description	Leucine-rich pentatricopeptide repeat containing
Protein class	Disease related genes Predicted intracellular proteins
Predicted localization	Intracellular
Number of transcripts	5
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HUMAN PROTEIN ATLAS INFORMATION
Summary	Localized to the mitochondria.
RNA cell category	Expressed in all
Protein evidence	Evidence at protein level
Main location	Localized to the Mitochondria (validated)
Single-cell variation	Single-cell variation in protein expression observed.
siRNA	The location(s) are Validated based on siRNA mediated gene silencing.

DATA RELIABILITY
Reliability score	Validated based on 2 antibodies. HPA036408 , HPA036409
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ASSAYS

RNA expression

Human cells

siRNA

RNA EXPRESSION OVERVIEW
RNA cell line category: Expressed in all Organ Origin Category Expression Alphabetical Cell lines sorted after organ of phenotypic resemblance. Cell lines sorted after biological source for establishment. Cell lines sorted after the cell line category according to Cellosaurus. Cell lines sorted on descending RNA expression. Cell lines sorted alphabetically.

HUMAN CELLS

Summary

Localized to the mitochondria.

Main location

Mitochondria (validated)


	Toggle channels	Low High G1 S G2 M N/A

Antibody	Cell line	Location	Single-cell variation
HPA036408	A-431	Mitochondria
HPA036408	U-2 OS	Mitochondria
HPA036408	U-251 MG	Mitochondria	Intensity
HPA036409	A-431	Mitochondria
HPA036409	U-2 OS	Mitochondria
HPA036409	U-251 MG	Mitochondria

siRNA VALIDATION

Relative fluorescence intensity (RFI)

Antibody

HPA036409

siRNA validation

Validated: Significant downregulation > 25% by both siRNA:s.

Analysis performed in

Cytoplasmic region of segmented cells in 10x-images

siRNA 1

siRNA 2

RFI

44%

57%

p-value

< 0.01


	Toggle channels	Low High G1 S G2 M N/A

Antibody	Sample	Cell line
HPA036409	siRNA 1	U-2 OS
HPA036409	siRNA 2	U-2 OS
HPA036409	scrambled	U-2 OS

GENE INFORMATION
Gene name	LRPPRC (HGNC Symbol)
Synonyms	GP130, LRP130, LSFC
Description	Leucine-rich pentatricopeptide repeat containing (HGNC Symbol)
Entrez gene summary	This gene encodes a leucine-rich protein that has multiple pentatricopeptide repeats (PPR). The precise role of this protein is unknown but studies suggest it may play a role in cytoskeletal organization, vesicular transport, or in transcriptional regulation of both nuclear and mitochondrial genes. The protein localizes primarily to mitochondria and is predicted to have an N-terminal mitochondrial targeting sequence. Mutations in this gene are associated with the French-Canadian type of Leigh syndrome. [provided by RefSeq, Mar 2012]
Chromosome	2
Cytoband	p21
Chromosome location (bp)	43886508 - 43996005
Protein evidence	Evidence at protein level (all genes)
Ensembl	ENSG00000138095 (version 83.38)
Entrez gene	10128
UniProt	P42704 (UniProt - Evidence at protein level)
neXtProt	NX_P42704
Antibodypedia	LRPPRC antibodies

PROTEIN BROWSER

LRPPRC-001	LRPPRC-002	LRPPRC-003	LRPPRC-005	LRPPRC-009

PROTEIN INFORMATION
Splice variant	UniProt	Protein class	Gene ontology	Length & mass	Signal peptide (predicted)	Transmembrane regions (predicted)
LRPPRC-001 ENSP00000260665 ENST00000260665	P42704 [Direct mapping] Leucine-rich PPR motif-containing protein, mitochondrial E5KNY5 [Target identity:100%; Query identity:100%] Leucine-rich PPR-motif containing; Mitochondrial leucine-rich PPR motif-containing protein Show all	Predicted intracellular proteins Disease related genes Protein evidence (Kim et al 2014) Protein evidence (Ezkurdia et al 2014) Show all	GO:0000794 [condensed nuclear chromosome] GO:0000961 [negative regulation of mitochondrial RNA catabolic process] GO:0003697 [single-stranded DNA binding] GO:0003723 [RNA binding] GO:0005515 [protein binding] GO:0005634 [nucleus] GO:0005637 [nuclear inner membrane] GO:0005640 [nuclear outer membrane] GO:0005654 [nucleoplasm] GO:0005737 [cytoplasm] GO:0005739 [mitochondrion] GO:0005856 [cytoskeleton] GO:0005874 [microtubule] GO:0006351 [transcription, DNA-templated] GO:0006355 [regulation of transcription, DNA-templated] GO:0008017 [microtubule binding] GO:0016020 [membrane] GO:0030529 [ribonucleoprotein complex] GO:0031625 [ubiquitin protein ligase binding] GO:0042645 [mitochondrial nucleoid] GO:0044822 [poly(A) RNA binding] GO:0047497 [mitochondrion transport along microtubule] GO:0048471 [perinuclear region of cytoplasm] GO:0048487 [beta-tubulin binding] GO:0051028 [mRNA transport] GO:0070129 [regulation of mitochondrial translation] Show all	1394 aa 157.9 kDa	No	0
LRPPRC-002 ENSP00000386234 ENST00000409946	A0A0C4DG06 [Direct mapping] Leucine-rich PPR motif-containing protein, mitochondrial Show all	Predicted intracellular proteins Protein evidence (Ezkurdia et al 2014) Show all		531 aa 59.3 kDa	No	0
LRPPRC-003 ENSP00000403637 ENST00000447246	C9JCA9 [Direct mapping] Leucine-rich PPR motif-containing protein, mitochondrial Show all	Predicted intracellular proteins Protein evidence (Ezkurdia et al 2014) Show all	GO:0005739 [mitochondrion] Show all	359 aa 40.1 kDa	No	0
LRPPRC-005 ENSP00000386562 ENST00000409659	B8ZZ38 [Direct mapping] Leucine-rich PPR motif-containing protein, mitochondrial Show all	Predicted intracellular proteins Protein evidence (Ezkurdia et al 2014) Show all	GO:0005739 [mitochondrion] Show all	504 aa 56.3 kDa	No	0
LRPPRC-009 ENSP00000414207 ENST00000419884	H7C3W8 [Direct mapping] Leucine-rich PPR motif-containing protein, mitochondrial Show all	Phobius predicted membrane proteins Predicted intracellular proteins Protein evidence (Ezkurdia et al 2014) Show all		77 aa 8.7 kDa	No	0