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CUZD1

ANTIBODY INFORMATION
 
 

Antibody HPA068479

  

Antibody HPA068660

  

Antibody HPA074117

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA068479   HPA068660   HPA074117  

Host species

 Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

      

Released in version

 14   14   16  

References

      

VALIDATION SUMMARY
 
 

Antibody HPA068479

  

Antibody HPA068660

  

Antibody HPA074117

  
Immunocytochemistry

    Approved  
Immunohistochemistry

Supported   Supported    
Western blot

Uncertain   Uncertain    
Protein array

Supported   Approved   Approved  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA068479

  

Antibody HPA068660

  

Antibody HPA074117

  

Standard validation

     Approved  

     

Figure description

     Immunofluorescent staining of human cell line RH-30 shows localization to nucleoli.  

Antibody dilution

     1:21  

Literature conformity

     The subcellular location is partly supported by literature or no literature is available.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA068479

  

Antibody HPA068660

  

Antibody HPA074117

  

Standard validation

 Supported   Supported    

     

Figure description

 Immunohistochemical staining of human pancreas shows moderate cytoplasmic positivity in exocrine glandular cells.   Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.    

Expression

 RNA: detected in 8 tissues
Protein: detected in 7 cell types		   RNA: detected in 8 tissues
Protein: detected in 11 cell types		    

Retrieval

 HIER pH6   HIER pH6    

Antibody dilution

 1:1200   1:800    

Literature conformity

 Consistent with gene/protein characterization data.   Consistent with gene/protein characterization data.    

RNA consistency

 Consistent with RNA expression data.   Consistent with RNA expression data.    

WESTERN BLOT
 
 

Antibody HPA068479

  

Antibody HPA068660

  

Antibody HPA074117

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		   Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		    

     

Figure description

      

Target mass (kDa)

 68.2, 40.2   68.2, 40.2    

Antibody dilution

 1:390   1:280    

PROTEIN ARRAY
 
 

Antibody HPA068479

  

Antibody HPA068660

  

Antibody HPA074117

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		  

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:4700   1:3300   1:550  

ANTIGEN INFORMATION
 
 

Antibody HPA068479

  

Antibody HPA068660

  

Antibody HPA074117

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 72   68   72  

Antigen sequence

 
QVSLRASDPNLVVFLDTCRASPTSDFASPTYDLIKSGCSRDETCKVYPLF
GHYGRFQFNAFKFLRSMSSVYL
  
CKVLICDSSDHQSRCNQGCVSRSKRDISSYKWKTDSIIGPIRLKRDRSAS
GNSGFQHETHAEETPNQP
  
SNGNNLQLKDPTCRPKLSNVVEFSVPLNGCGTIRKVEDQSITYTNIITFS
ASSTSEVITRQKQLQIIVKCEM
 

Matching transcripts

 CUZD1-001 - ENSP00000357900 [97%]
CUZD1-201 - ENSP00000376540 [97%]
CUZD1-202 - ENSP00000441590 [97%]
  CUZD1-001 - ENSP00000357900 [100%]
CUZD1-201 - ENSP00000376540 [100%]
CUZD1-202 - ENSP00000441590 [100%]
  CUZD1-001 - ENSP00000357900 [100%]
CUZD1-201 - ENSP00000376540 [100%]
CUZD1-202 - ENSP00000441590 [100%]
 

Other gene match

      
ANTIGEN VIEW
CUZD1-001
CUZD1-201
CUZD1-202
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.