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FAM117B

ANTIBODY INFORMATION
 
 

Antibody HPA003229

  

Antibody HPA028746

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA003229   HPA028746  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 3   13  

References

 2    

VALIDATION SUMMARY
 
 

Antibody HPA003229

  

Antibody HPA028746

  
Immunocytochemistry

  Approved  
Immunohistochemistry

Uncertain   Uncertain  
Western blot

Uncertain   Uncertain  
Protein array

Supported   Approved  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA003229

  

Antibody HPA028746

  

Standard validation

   Approved  

   

Figure description

   Immunofluorescent staining of human cell line SiHa shows localization to nucleus & centrosome.  

Antibody dilution

   1:109  

Literature conformity

   The subcellular location is partly supported by literature or no literature is available.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA003229

  

Antibody HPA028746

  

Standard validation

 Uncertain   Uncertain  

   

Figure description

 Immunohistochemical staining of human pancreas shows distinct cytoplasmic positivity in exocrine glandular cells.   Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.  

Expression

 RNA: detected in 35 tissues
Protein: detected in 32 cell types		   RNA: detected in 35 tissues
Protein: detected in 29 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:150   1:50  

Literature conformity

 No avaliable gene/protein characterization data.   No avaliable gene/protein characterization data.  

RNA consistency

 Mainly not consistent with RNA expression data.   Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA003229

  

Antibody HPA028746

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		  

   

Figure description

    

Target mass (kDa)

 62   62  

Antibody dilution

 1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA003229

  

Antibody HPA028746

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:6000  

ANTIGEN INFORMATION
 
 

Antibody HPA003229

  

Antibody HPA028746

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 107   123  

Antigen sequence

 
ECSPKQLHEIPAFYCPDKNKVNFIPKSGSAFCLVSILKPLLPTPDLTLKG
SGHSLTVTTGMTTTLLQPIAVASLSTNTEQDRVSRGTSTVMPSASLLPPP
EPIEEAE
  
LLGTVSSPSSSPTHLWTGEVSAAPPPARVRHRRRSPEQSRSSPEKRSPSA
PVCKAGDKTRQPSSSPSSIIRRTSSLDTLAAPYLAGHWPRDSHGQAAPCM
RDKATQTESAWAEEYSEKKKGSH
 

Matching transcripts

 FAM117B-001 - ENSP00000376071 [100%]
  FAM117B-001 - ENSP00000376071 [100%]
 

Other gene match

    
ANTIGEN VIEW
FAM117B-001

RELEVANT PUBLICATIONS
 
 

Antibody HPA003229

  

Antibody HPA028746

  

1.

 Expression profiling of microdissected cell populations selected from basal cells in normal epidermis and basal cell carcinoma
Asplund A et al
Br J Dermatol 2008;158(3):527-38
    

2.

 Tissue profiling of the mammalian central nervous system using human antibody-based proteomics
Mulder J et al
Mol Cell Proteomics 2009;8(7):1612-22
    
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.