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Immunofluorescent staining of human cell line U-2 OS shows localization to the Golgi apparatus.
Antibody dilution
1:93
Literature conformity
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA035863
Antibody HPA058115
Standard validation
Approved
Figure description
Immunohistochemical staining of human placenta shows cytoplasmic positivity in trophoblasts.
Expression
RNA: detected in 37 tissues Protein: detected in 66 cell types
Retrieval
HIER pH6
Antibody dilution
1:300
Literature conformity
Partly consistent with gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA035863
Antibody HPA058115
Standard validation
Uncertain
Single band larger than predicted size in kDa (+20%) but partly supported by experimental and/or bioinformatic data.
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY413001)
Target mass (kDa)
34.3, 30.5
34.3, 30.5, 8.4
Antibody dilution
1:250
1:310
PROTEIN ARRAY
Antibody HPA035863
Antibody HPA058115
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
