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Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear speckles.
Antibody dilution
1:50
Literature conformity
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA026436
Antibody HPA026451
Antibody HPA026501
Standard validation
Approved
Approved
Approved
Figure description
Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity with a granular pattern in exocrine and endocrine cells.
Immunohistochemical staining of human duodenum shows strong cytoplasmic and nuclear positivity in glandular cells.
Immunohistochemical staining of human placenta shows strong cytoplasmic positivity in decidual cells.
Expression
RNA: detected in 35 tissues Protein: detected in 80 cell types
RNA: detected in 35 tissues Protein: detected in 79 cell types
RNA: detected in 35 tissues Protein: detected in 22 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:175
1:50
1:600
Literature conformity
Not consistent with gene/protein characterization data.
Not consistent with gene/protein characterization data.
Not consistent with gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
Mainly consistent with RNA expression data.
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA026436
Antibody HPA026451
Antibody HPA026501
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Figure description
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
129.2, 95.5
129.2, 95.5, 73.8
129.2, 95.5
Antibody dilution
1:250
1:250
1:250
PROTEIN ARRAY
Antibody HPA026436
Antibody HPA026451
Antibody HPA026501
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
