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GSTCD

ANTIBODY INFORMATION
 
 

Antibody HPA046160

  

Antibody HPA061782

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA046160   HPA061782  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 9   14  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA046160

  

Antibody HPA061782

  
Immunocytochemistry


Validated
  

Validated
  
Immunohistochemistry

Approved    
Western blot

Uncertain   Uncertain  
Protein array

Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA046160

  

Antibody HPA061782

  

Formal validation: Independent
Validated

Antibody staining overlaps with antibody HPA061782.		  
Validated

Antibody staining overlaps with antibody HPA046160.		  
 

Standard validation

 Uncertain   Uncertain  

   

Figure description

 Immunofluorescent staining of human cell line U-251 MG shows localization to nucleus.   Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.  

Antibody dilution

 1:10   1:4  

Literature conformity

 The subcellular location is not consistent with literature.   The subcellular location is not consistent with literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA046160

  

Antibody HPA061782

  

Standard validation

 Approved    

   

Figure description

 Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells in tubules.    

Expression

 RNA: detected in 34 tissues
Protein: detected in 61 cell types		    

Retrieval

 HIER pH6    

Antibody dilution

 1:15    

Literature conformity

 No avaliable gene/protein characterization data.    

RNA consistency

 Mainly not consistent with RNA expression data.    

WESTERN BLOT
 
 

Antibody HPA046160

  

Antibody HPA061782

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		  

   

Figure description

   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 71.1, 61.3   71.1, 61.3, 38.2  

Antibody dilution

 1:100   1:80  

PROTEIN ARRAY
 
 

Antibody HPA046160

  

Antibody HPA061782

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:800   1:500  

ANTIGEN INFORMATION
 
 

Antibody HPA046160

  

Antibody HPA061782

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 85   97  

Antigen sequence

 
TRASFVTCPCCYGFIQNTSKFNFPKSEQFKKTLSYKEHMILCRFADQTAV
QLPPQRRLIGKQCMCLVDLDRARAAEECGYSVQVI
  
SEKLVEFPLLASWYQRIQEVPGVKTAASKCGIQFLHLPKLLTTSTEQHPN
LCEVPGVEEQSDPLFIGGPRPTMAKLMEKGIEVMFSPHPCPTWTLDW
 

Matching transcripts

 GSTCD-001 - ENSP00000378218 [100%]
GSTCD-002 - ENSP00000353695 [100%]
GSTCD-006 - ENSP00000422354 [100%]
GSTCD-012 - ENSP00000378216 [100%]
  GSTCD-001 - ENSP00000378218 [100%]
GSTCD-002 - ENSP00000353695 [100%]
GSTCD-005 - ENSP00000422858 [100%]
GSTCD-006 - ENSP00000422354 [100%]
GSTCD-012 - ENSP00000378216 [100%]
 

Other gene match

    
ANTIGEN VIEW
GSTCD-001
GSTCD-002
GSTCD-005
GSTCD-006
GSTCD-012
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.