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Antibody staining overlaps with antibody HPA022282.
Validated
Antibody staining overlaps with antibody HPA020893.
Standard validation
Supported
Supported
Figure description
Immunofluorescent staining of human cell line RH-30 shows localization to nucleus.
Immunofluorescent staining of human cell line RH-30 shows localization to nucleus.
Antibody dilution
1:31
1:55
Literature conformity
The subcellular location is supported by literature.
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA020893
Antibody HPA022282
Standard validation
Uncertain
Uncertain
Figure description
Immunohistochemical staining of human duodenum shows distinct positivity in subsets of leukocytes
Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.
Expression
RNA: detected in 37 tissues Protein: detected in 4 cell types
RNA: detected in 37 tissues Protein: detected in 33 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:10
1:35
Literature conformity
No avaliable gene/protein characterization data.
No avaliable gene/protein characterization data.
RNA consistency
Not consistent with RNA expression data.
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA020893
Antibody HPA022282
Standard validation
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY403504)
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
54.5, 52.8, 34, 30.7, 11.9
54.5, 52.8, 34, 30.7, 16.2, 15
Antibody dilution
1:125
1:250
PROTEIN ARRAY
Antibody HPA020893
Antibody HPA022282
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
