Cell atlas - ETFA - The Human Protein Atlas

GENERAL INFORMATION
Gene name	ETFA
Gene description	Electron-transfer-flavoprotein, alpha polypeptide
Protein class	Disease related genes Plasma proteins Predicted intracellular proteins
Predicted localization	Intracellular
Number of transcripts	8
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HUMAN PROTEIN ATLAS INFORMATION
Summary	Localized to the mitochondria.
RNA cell category	Expressed in all
Protein evidence	Evidence at protein level
Main location	Localized to the Mitochondria (validated)
Single-cell variation	Single-cell variation in protein expression observed.
siRNA	The location(s) are Validated based on siRNA mediated gene silencing.

DATA RELIABILITY
Reliability score	Validated based on 3 antibodies. HPA018990 , HPA018993 , HPA024089
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ASSAYS

RNA expression

Human cells

siRNA

RNA EXPRESSION OVERVIEW
RNA cell line category: Expressed in all Organ Origin Category Expression Alphabetical Cell lines sorted after organ of phenotypic resemblance. Cell lines sorted after biological source for establishment. Cell lines sorted after the cell line category according to Cellosaurus. Cell lines sorted on descending RNA expression. Cell lines sorted alphabetically.

HUMAN CELLS

Summary

Localized to the mitochondria.

Main location

Mitochondria (validated)


	Toggle channels	Low High G1 S G2 M N/A

Antibody	Cell line	Location	Single-cell variation
HPA018990	A-431	Mitochondria
HPA018990	U-2 OS	Mitochondria
HPA018990	U-251 MG	Mitochondria
HPA018993	A-431	Mitochondria
HPA018993	U-2 OS	Mitochondria
HPA018993	U-251 MG	Mitochondria	Intensity
HPA024089	A-431	Mitochondria
HPA024089	U-2 OS	Mitochondria
HPA024089	U-251 MG	Mitochondria

siRNA VALIDATION

Relative fluorescence intensity (RFI)

Antibody

HPA024089

siRNA validation

Validated: Significant downregulation > 25% by both siRNA:s.

Analysis performed in

Whole cells of segmented cells in 10x-images

siRNA 1

siRNA 2

RFI

42%

50%

p-value

< 0.01


	Toggle channels	Low High G1 S G2 M N/A

Antibody	Sample	Cell line
HPA024089	siRNA 1	U-2 OS
HPA024089	siRNA 2	U-2 OS
HPA024089	scrambled	U-2 OS

GENE INFORMATION
Gene name	ETFA (HGNC Symbol)
Synonyms	EMA, GA2, MADD
Description	Electron-transfer-flavoprotein, alpha polypeptide (HGNC Symbol)
Entrez gene summary	ETFA participates in catalyzing the initial step of the mitochondrial fatty acid beta-oxidation. It shuttles electrons between primary flavoprotein dehydrogenases and the membrane-bound electron transfer flavoprotein ubiquinone oxidoreductase. Defects in electron-transfer-flavoprotein have been implicated in type II glutaricaciduria in which multiple acyl-CoA dehydrogenase deficiencies result in large excretion of glutaric, lactic, ethylmalonic, butyric, isobutyric, 2-methyl-butyric, and isovaleric acids. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Chromosome	15
Cytoband
Chromosome location (bp)	76215355 - 76311472
Protein evidence	Evidence at protein level (all genes)
Ensembl	ENSG00000140374 (version 83.38)
Entrez gene	2108
UniProt	P13804 (UniProt - Evidence at protein level)
neXtProt	NX_P13804
Antibodypedia	ETFA antibodies

PROTEIN BROWSER

ETFA-001	ETFA-002	ETFA-006	ETFA-011	ETFA-012	ETFA-014	ETFA-017	ETFA-019

PROTEIN INFORMATION
Splice variant	UniProt	Protein class	Gene ontology	Length & mass	Signal peptide (predicted)	Transmembrane regions (predicted)
ETFA-001 ENSP00000452762 ENST00000557943	P13804 [Direct mapping] Electron transfer flavoprotein subunit alpha, mitochondrial Show all	Predicted intracellular proteins Plasma proteins Disease related genes Protein evidence (Kim et al 2014) Protein evidence (Ezkurdia et al 2014) Show all	GO:0000062 [fatty-acyl-CoA binding] GO:0003995 [acyl-CoA dehydrogenase activity] GO:0005515 [protein binding] GO:0005739 [mitochondrion] GO:0005759 [mitochondrial matrix] GO:0009055 [electron carrier activity] GO:0016491 [oxidoreductase activity] GO:0022904 [respiratory electron transport chain] GO:0033539 [fatty acid beta-oxidation using acyl-CoA dehydrogenase] GO:0044237 [cellular metabolic process] GO:0044281 [small molecule metabolic process] GO:0050660 [flavin adenine dinucleotide binding] GO:0052890 [oxidoreductase activity, acting on the CH-CH group of donors, with a flavin as acceptor] GO:0055088 [lipid homeostasis] GO:0070062 [extracellular exosome] Show all	333 aa 35.1 kDa	No	0
ETFA-002 ENSP00000399273 ENST00000433983	P13804 [Direct mapping] Electron transfer flavoprotein subunit alpha, mitochondrial Show all	Predicted intracellular proteins Plasma proteins Disease related genes Protein evidence (Kim et al 2014) Protein evidence (Ezkurdia et al 2014) Show all	GO:0000062 [fatty-acyl-CoA binding] GO:0003995 [acyl-CoA dehydrogenase activity] GO:0005739 [mitochondrion] GO:0005759 [mitochondrial matrix] GO:0009055 [electron carrier activity] GO:0016491 [oxidoreductase activity] GO:0022904 [respiratory electron transport chain] GO:0033539 [fatty acid beta-oxidation using acyl-CoA dehydrogenase] GO:0044237 [cellular metabolic process] GO:0044281 [small molecule metabolic process] GO:0050660 [flavin adenine dinucleotide binding] GO:0052890 [oxidoreductase activity, acting on the CH-CH group of donors, with a flavin as acceptor] GO:0055088 [lipid homeostasis] GO:0070062 [extracellular exosome] Show all	284 aa 30 kDa	No	0
ETFA-006 ENSP00000453098 ENST00000560726	H0YL83 [Direct mapping] Electron transfer flavoprotein subunit alpha, mitochondrial Show all	Predicted intracellular proteins Protein evidence (Ezkurdia et al 2014) Show all	GO:0005739 [mitochondrion] Show all	73 aa 7.8 kDa	No	0
ETFA-011 ENSP00000452777 ENST00000559386	H0YKF0 [Direct mapping] Electron transfer flavoprotein subunit alpha, mitochondrial Show all	SCAMPI predicted membrane proteins Predicted intracellular proteins Protein evidence (Ezkurdia et al 2014) Show all	GO:0005739 [mitochondrion] Show all	289 aa 30.2 kDa	No	0
ETFA-012 ENSP00000453017 ENST00000560345	H0YL12 [Direct mapping] Electron transfer flavoprotein subunit alpha, mitochondrial Show all	SCAMPI predicted membrane proteins Predicted intracellular proteins Protein evidence (Ezkurdia et al 2014) Show all		240 aa 24.9 kDa	No	0
ETFA-014 ENSP00000452659 ENST00000559602	H0YK49 [Direct mapping] Electron transfer flavoprotein subunit alpha, mitochondrial Show all	Predicted intracellular proteins Protein evidence (Ezkurdia et al 2014) Show all	GO:0005739 [mitochondrion] GO:0009055 [electron carrier activity] GO:0050660 [flavin adenine dinucleotide binding] Show all	229 aa 24.2 kDa	No	0
ETFA-017 ENSP00000454194 ENST00000559973	H0YNX6 [Direct mapping] Electron transfer flavoprotein subunit alpha, mitochondrial Show all	Predicted intracellular proteins Protein evidence (Ezkurdia et al 2014) Show all	GO:0009055 [electron carrier activity] GO:0050660 [flavin adenine dinucleotide binding] Show all	254 aa 26.8 kDa	No	0
ETFA-019 ENSP00000453345 ENST00000560595	H0YLU7 [Direct mapping] Electron transfer flavoprotein subunit alpha, mitochondrial Show all	Predicted intracellular proteins Protein evidence (Ezkurdia et al 2014) Show all	GO:0005739 [mitochondrion] GO:0009055 [electron carrier activity] GO:0050660 [flavin adenine dinucleotide binding] Show all	303 aa 31.7 kDa	No	0