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RLBP1

ANTIBODY INFORMATION
 
 

Antibody HPA042051

  

Antibody HPA044083

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA042051   HPA044083  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 16   16  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA042051

  

Antibody HPA044083

  
Immunocytochemistry

  Approved  
Western blot

Uncertain   Uncertain  
Protein array

Approved   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA042051

  

Antibody HPA044083

  

Standard validation

   Approved  

   

Figure description

   Immunofluorescent staining of human cell line SK-MEL-30 shows localization to nucleoplasm, cytosol & centrosome.  

Antibody dilution

   1:105  

Literature conformity

   The subcellular location is partly supported by literature or no literature is available.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA042051

  

Antibody HPA044083

  

Standard validation

 Approved    

   

Figure description

 Positivity observed in microglia, in all brain regions.    

WESTERN BLOT
 
 

Antibody HPA042051

  

Antibody HPA044083

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		   Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		  

   

Figure description

    

Target mass (kDa)

 36.5   36.5, 12.1  

Antibody dilution

 1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA042051

  

Antibody HPA044083

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:12000  

ANTIGEN INFORMATION
 
 

Antibody HPA042051

  

Antibody HPA044083

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 97   57  

Antigen sequence

 
TTKDHGPVFGPCSQLPRHTLQKAKDELNEREETREEAVRELQEMVQAQAA
SGEELAVAVAERVQEKDSGFFLRFIRARKFNVGRAYELLRGYVNFRL
  
PARFKAIHFIHQPWYFTTTYNVVKPFLKSKLLERVFVHGDDLSGFYQEID
ENILPSD
 

Matching transcripts

 RLBP1-001 - ENSP00000268125 [100%]
  RLBP1-001 - ENSP00000268125 [100%]
RLBP1-005 - ENSP00000454740 [81%]
 

Other gene match

    
ANTIGEN VIEW
RLBP1-001
RLBP1-005
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.