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PMM2

ANTIBODY INFORMATION
 
 

Antibody HPA040852

  

Antibody HPA063649

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA040852   HPA063649  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 8   14  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA040852

  

Antibody HPA063649

  
Immunocytochemistry

Uncertain   Supported  
Western blot

Supported   Supported  
Protein array

Approved   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA040852

  

Antibody HPA063649

  

Standard validation

 Uncertain   Supported  

   

Figure description

 Immunofluorescent staining of human cell line U-2 OS shows localization to nucleus.   Immunofluorescent staining of human cell line SH-SY5Y shows localization to nucleus & cytosol.  

Antibody dilution

 1:52   1:53  

Literature conformity

 The subcellular location is not consistent with literature.   The subcellular location is supported by literature.  

WESTERN BLOT
 
 

Antibody HPA040852

  

Antibody HPA063649

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

   

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 28.1, 25.3, 17.5   28.1, 25.3  

Antibody dilution

 1:250   1:180  

PROTEIN ARRAY
 
 

Antibody HPA040852

  

Antibody HPA063649

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:2100  

ANTIGEN INFORMATION
 
 

Antibody HPA040852

  

Antibody HPA063649

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 60   41  

Antigen sequence

 
WDKRYCLRHVENDGYKTIYFFGDKTMPGGNDHEIFTDPRTMGYSVTAPED
TRRICELLFS
  
FEKVQEQLGNDVVEKYDYVFPENGLVAYKDGKLLCRQNIQS
 

Matching transcripts

 PMM2-001 - ENSP00000268261 [100%]
PMM2-015 - ENSP00000456302 [100%]
PMM2-018 - ENSP00000457956 [100%]
  PMM2-001 - ENSP00000268261 [100%]
PMM2-018 - ENSP00000457956 [100%]
 

Other gene match

    
ANTIGEN VIEW
PMM2-001
PMM2-015
PMM2-018
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.